The primary plasminogen-activator inhibitors in endothelial cells, platelets, serum, and plasma are immunologically related.
The primary plasminogen-activator inhibitors in endothelial cells, platelets, serum, and plasma are immunologically related.
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内皮细胞、血小板、血清和血浆中的主要纤溶酶原激活剂抑制剂与免疫学相关。
DOI：
10.1073/pnas.82.24.8710
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发表时间：
1985
影响因子：
11.1
通讯作者：
Loskutoff,DJ
中科院分区：
文献类型：
--
作者：
Erickson,LA;Hekman,CM;Loskutoff,DJ
从培养的牛主动脉内皮细胞中纯化出一种非常稳定的Mr50，000纤溶酶原激活物抑制剂（派）的单特异性抗血清，结合反向纤维蛋白自显影术，以确定人血小板、血清和血浆中是否含有免疫相关的抑制剂。反向纤维蛋白自显影显示血小板和血清样本中存在Mr 50，000抑制剂，但正常血浆中不存在。然而，从派活性增加的个体获得的血浆中检测到Mr 50，000抑制剂。在每种情况下，用抗抑制剂血清处理样品去除Mr 50，000抑制剂。存在于每个样品中的抑制剂以快速的方式中和外源性添加的组织型纤溶酶原激活剂。抑制与形成的NaDodSO4抗性酶抑制剂复合物的先生120，000。同样，用抗抑制剂血清处理样品去除了抑制活性和这些样品中与组织型纤溶酶原激活剂结合的组分。因此，存在于人血小板、血清和患者血浆中的快速作用的派与由培养的牛主动脉内皮细胞合成的派在免疫学上相关。该分子可能是血管系统中纤溶酶原激活物的生理相关抑制剂，因此，可能在调节血管纤维蛋白溶解的起始中起重要作用。
Monospecific antiserum to an unusually stable Mr 50,000 plasminogen-activator inhibitor (PAI) purified from cultured bovine aortic endothelial cells was employed in conjunction with reverse fibrin autography to determine whether human platelets, serum, and plasma contain immunologically related inhibitors. Reverse fibrin autography revealed the presence of a Mr 50,000 inhibitor in the platelet and serum samples but not in normal plasma. However, a Mr 50,000 inhibitor was detected in plasma obtained from individuals with increased PAI activity. In each case, treatment of the sample with the anti-inhibitor serum removed the Mr 50,000 inhibitor. The inhibitor present in each sample neutralized exogenously added tissue-type plasminogen activator in a rapid manner. Inhibition was associated with the formation of a NaDodSO4-resistant enzyme-inhibitor complex of Mr 120,000. Again, treatment of the samples with the anti-inhibitor serum removed both the inhibitory activity and the component in these samples that binds to tissue-type plasminogen activator. Thus, the rapidly acting PAI present in human platelets, serum, and patient plasma is immunologically related to the PAI synthesized by cultured bovine aortic endothelial cells. This molecule may be the physiologically relevant inhibitor of plasminogen activator in the vascular system and, as such, may serve an important role in regulating the initiation of vascular fibrinolysis.