Densitometry, radiography, and histological assessment of collagen as methods to evaluate femoral bones in an experimental model of osteoporosis
Densitometry, radiography, and histological assessment of collagen as methods to evaluate femoral bones in an experimental model of osteoporosis
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DOI：
10.1007/s00198-011-1539-8
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发表时间：
2012-02-01
影响因子：
4
通讯作者：
Salazar, M.
中科院分区：
文献类型：
--
作者：
Hernandes, L.;Ramos, A. L.;Salazar, M.
所有检测实验性骨丢失的方法都存在技术局限性。评价了图像和组织学分析的灵敏度，以检测卵巢切除术后骨丢失大鼠中特立哌酮的作用。卵巢切除术后骨丢失评估方法的标准化对于建立实验性骨质疏松的程度至关重要。通常，使用每图像或组织学技术的方法。为了验证这两种方法来确定骨质流失的程度在卵巢切除大鼠，我们评估了骨密度测定法，传统的X线摄影，和组织学分析的胶原蛋白所占的面积的敏感性，检测特立哌酮治疗的影响，在股骨的卵巢切除大鼠骨流失。卵巢切除组，其中进行双侧卵巢的切除;和卵巢切除+特立哌利组，其中进行双侧卵巢的切除，并且用3 μ g/100 g/天的特立哌利治疗动物。卵巢切除后3个月，对股骨干进行骨密度测定、X线骨密度测定和胶原纤维所占面积的组织学分析，骨密度测定显示股骨密度降低11.2%;常规X线片显示骨量丢失14.5%，组织学分析显示，在股骨干中检测到胶原蛋白所占面积减少40.9%。总之，组织学分析不能与骨密度测定法和常规X线摄影法进行定量比较;然而，所有这些方法对于评估卵巢切除术引起的骨丢失和特立哌酮预防骨质疏松症的治疗效果都是定性有效的。
All methods to detect experimental loss of bone present technique limitations. The sensitivities of image and histological analyses to detect the effects of teriparatide in rats with bone loss after ovariectomy were evaluated. All methods were qualitatively valid.The standardization of methods to assess bone loss after ovariectomy is crucial to establish the degree of experimental osteoporosis. In general, methods per image or histological techniques are used. To validate these two ways to determine the degree of bone loss in ovariectomized rats, we evaluated the sensitivities of bone densitometry, conventional radiography, and histological analysis of the area occupied by collagen, detecting the effects of teriparatide treatment in the femur of ovariectomized rats with bone loss.Wistar rats were divided into three groups: a control group, in which the animals were only subjected to laparotomy; an ovariectomized group, in which bilateral removal of the ovaries was performed; and an ovariectomized + teriparatide group, in which bilateral removal of the ovaries was performed, and the animals were treated with 3 mu g/100 g/day of teriparatide. Three months following the ovariectomy, bone densitometry, radiographic densitometry, and histological analysis of the area occupied by collagen fibers were carried out in the femur diaphysis.The bone densitometry revealed 11.2% reduction in femur density; in the conventional radiography, the loss of bone mass was 14.5%, and with the histological analysis, a 40.9% reduction in the area occupied by collagen was detected in the femur diaphysis.In conclusion, histological analysis could not be quantitatively compared with the methods of bone densitometry and conventional radiography; however, all of these methods were qualitatively valid for assessing the loss of bone stemming from ovariectomy and the therapeutic effect of teriparatide in the prevention of osteoporosis.