Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections.
Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections.
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DOI：
10.1128/mbio.00231-13
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发表时间：
2013-06-18
期刊：
影响因子：
6.4
通讯作者：
de Jong MD
中科院分区：
文献类型：
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作者：
Tan le V;van Doorn HR;Nghia HD;Chau TT;Tu le TP;de Vries M;Canuti M;Deijs M;Jebbink MF;Baker S;Bryant JE;Tham NT;BKrong NT;Boni MF;Loi TQ;Phuong le T;Verhoeven JT;Crusat M;Jeeninga RE;Schultsz C;Chau NV;Hien TT;van der Hoek L;Farrar J;de Jong MD
急性中枢神经系统（CNS）感染导致大量的发病率和死亡率，但在很大比例的情况下，病因仍然未知。我们在两名病因不明的越南CNS感染患者的脑脊液（CSF）标本中鉴定并鉴定了一种新型环状病毒（暂命名为越南环状病毒[CyCV-VN]）的全基因组。CyCV-VN随后在疑似CNS感染的越南患者的642份CSF标本中的4%中检测到，而在非感染性神经系统疾病患者的122份CSF中没有检测到。在不明病因的CNS感染患者和检测到其他病原体的患者中，检出率相似。健康儿童粪便中的类似检出率表明食源性或口粪传播途径，而猪和家禽粪便中的高检出率（平均为58%）表明存在此类传播的动物宿主。需要进一步的研究来解决这种新的、潜在的人畜共患病病毒的流行病学和致病性。急性中枢神经系统（CNS）感染导致大量的发病率和死亡率，但病因往往仍然不明，这阻碍了治疗或预防策略的发展。因此，新病原体的鉴定是必不可少的，并且通过当前基于测序的下一代方法来促进。使用这种技术，我们确定并表征了一种新型环状病毒的全基因组的脑脊液（CSF）标本从两个越南患者的CNS感染的病因不明，随后检测到没有122例CSF标本从非感染性神经系统疾病的患者，但4%的642例CSF标本从越南患者疑似或确诊的CNS感染。健康儿童粪便中的类似检出率表明食源性或口粪传播途径，而越南猪和家禽粪便中的频繁检出（平均58%）表明存在此类传播的动物宿主。需要进一步的研究来解决这种新的、潜在的人畜共患病病毒的流行病学和致病性。
Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology remains unknown in a large proportion of cases. We identified and characterized the full genome of a novel cyclovirus (tentatively named cyclovirus-Vietnam [CyCV-VN]) in cerebrospinal fluid (CSF) specimens of two Vietnamese patients with CNS infections of unknown etiology. CyCV-VN was subsequently detected in 4% of 642 CSF specimens from Vietnamese patients with suspected CNS infections and none of 122 CSFs from patients with noninfectious neurological disorders. Detection rates were similar in patients with CNS infections of unknown etiology and those in whom other pathogens were detected. A similar detection rate in feces from healthy children suggested food-borne or orofecal transmission routes, while high detection rates in feces from pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further research is needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus. Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology frequently remains unknown, which hampers development of therapeutic or preventive strategies. Hence, identification of novel pathogens is essential and is facilitated by current next-generation sequencing-based methods. Using such technology, we identified and characterized the full genome of a novel cyclovirus in cerebrospinal fluid (CSF) specimens from two Vietnamese patients with CNS infections of unknown etiology, which was subsequently detected in none of 122 CSF specimens from patients with noninfectious neurological disorders but 4% of 642 CSF specimens from Vietnamese patients with suspected or confirmed CNS infections. Similar detection rates in feces from healthy children suggested food-borne or orofecal transmission routes, while frequent detection in feces from Vietnamese pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further studies are needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus.