Hepatitis C Virus Seroprevalence in Mongolian Women Assessed by a Novel Multiplex Antibody Detection Assay
Hepatitis C Virus Seroprevalence in Mongolian Women Assessed by a Novel Multiplex Antibody Detection Assay
复制标题
DOI：
10.1158/1055-9965.epi-15-0351
复制
发表时间：
2015-09-01
影响因子：
3.8
通讯作者：
Waterboer, Tim
中科院分区：
文献类型：
--
作者：
Dondog, Bolormaa;Schnitzler, Paul;Waterboer, Tim
背景：丙型肝炎病毒（HCV）感染导致肝细胞癌，是工业化国家和发展中国家死亡的重要原因。我们开发了一种用于HCV抗体检测的单步高通量多重血清学检测方法，并确定了HCV在一个高流行国家的流行情况。方法：重组表达HCV三种最常见亚型（1a、1b、2a）的5种蛋白（Core、NS3、NS4A、NS5A、NS5B），并将其作为多重抗体检测试验的抗原。采用商用ELISA、Western blot和RNA检测确定HCV状态的432份参考血清验证多重HCV血清学。在1023份代表蒙古成年女性人群的血清中检测了HCV抗体。结果：在参考血清中，多重血清学检测HCV（主要是Core和NS3）抗体的灵敏度为100%，特异性为99.6%，与商业诊断方法非常吻合（kappa为0.96；95%可信区间为0.92-0.99）。NS4和NS5抗体的作用仍有待评估。在蒙古，总的HCV抗体流行率为18.9%（按世界人口年龄标准化后为17.8%）。HCV血清患病率随着年龄的增长而增加，从30岁以下女性的10%增加到50岁以下女性的32%，但与性危险因素无关。结论：单步高通量多重HCV血清学检测与常规HCV抗体筛选后进行二次确认试验的效果相似。经证实，蒙古女性人口中所有社会经济群体的HCV血清患病率都非常高。影响：多重HCV血清学有助于HCV感染的大规模血清流行病学研究。(c) 2015年aacr。
Background: Hepatitis C virus (HCV) infection causes hepatocellular carcinoma and is an important cause of mortality in both industrialized and developing countries. We developed a single-step high-throughput multiplex serology assay for HCV antibody detection and determined HCV prevalence in a highly endemic country.Methods: Five proteins (Core, NS3, NS4A, NS5A, NS5B) each from the three most common subtypes of HCV (1a, 1b, 2a) were recombinantly expressed and used as antigens in a multiplexed antibody detection assay. Multiplex HCV serology was validated with 432 reference sera whose HCV status was established by commercial ELISA, Western blot, and RNA assays. HCV antibodies were determined in 1,023 sera representative for the adult female population of Mongolia.Results: In reference sera, detection of HCV (mostly Core and NS3) antibodies by multiplex serology showed 100% sensitivity and 99.6% specificity, and was in very good agreement with the commercial diagnostic assays (kappa, 0.96; 95% confidence interval, 0.92-0.99). The role of antibodies to NS4 and NS5 remains to be evaluated. In Mongolia, overall HCV antibody prevalence was 18.9% (17.8% when age-standardized to the world population). HCV seroprevalence increased with age from 10% in women < 30 years to 32% in women >= 50 years, but was not related to sexual risk factors.Conclusions: The single-step high-throughput multiplex HCV serology assay performs similarly to conventional HCV antibody screening followed by secondary confirmation assays. A very high HCV seroprevalence was confirmed across all socio-economic groups in the female population of Mongolia.Impact: Multiplex HCV serology facilitates large seroepidemiologic studies of HCV infection. (C) 2015 AACR.