Slow-freezing versus vitrification for human ovarian tissue cryopreservation
Slow-freezing versus vitrification for human ovarian tissue cryopreservation
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DOI：
10.1007/s00404-014-3390-6
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发表时间：
2015-02-01
影响因子：
2.6
通讯作者：
Griesinger, Georg
中科院分区：
文献类型：
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作者：
Klocke, Silke;Buendgen, Nana;Griesinger, Georg
卵巢组织可以在化疗前使用缓慢冷冻或玻璃化方法进行冷冻保存;然而，关于程序的平等性的数据仍然相互矛盾。本研究比较了玻璃化冷冻和慢速冷冻对人卵巢组织的冷冻损伤，对23例绝经前患者的卵巢组织进行了慢速冷冻和玻璃化冷冻。解冻/加温后，对组织进行组织学和免疫化学分析并进行体外培养。在组织培养过程中，雌二醇的释放进行了评估。在解冻/升温后，在缓慢冷冻和玻璃化冷冻组中，高质量卵泡的比例分别为72.7%和66.7%，p = 0.733，无显著差异。在18天的体外培养中，两组卵巢组织的雌二醇分泌相似（曲线下面积分别为5，411和13，102，p = 0.11）。添加鞘氨醇-1-磷酸或激活素A的培养基中没有改变两组雌二醇的释放。在培养期结束时，慢速冷冻和玻璃化冷冻的活化Caspase-3或“增殖细胞核抗原”阳性卵泡的比例相似。慢速冷冻和玻璃化冷冻导致冷冻保存后相似的形态完整性，培养中相似的雌二醇释放，以及培养后相似的卵泡增殖和凋亡率。
Ovarian tissue can be cryopreserved prior to chemotherapy using either the slow-freezing or the vitrification method; however, the data on the equality of the procedures are still conflicting. In this study, a comparison of the cryo-damage of human ovarian tissue induced by either vitrification or slow-freezing was performed.Ovarian tissue from 23 pre-menopausal patients was cryopreserved with either slow-freezing or vitrification. After thawing/warming, the tissue was histologically and immunohistochemically analyzed and cultured in vitro. During tissue culture the estradiol release was assessed.No significant difference was found in the proportion of high-quality follicles after thawing/warming in the slow-freezing and vitrification group, respectively (72.7 versus 66.7 %, p = 0.733). Estradiol secretion by the ovarian tissue was similar between groups during 18 days in vitro culture (area-under-the-curve 5,411 versus 13,102, p = 0.11). Addition of Sphingosine-1-Phosphate or Activin A to the culture medium did not alter estradiol release in both groups. The proportion of Activated Caspase-3 or 'Proliferating-Cell-Nuclear-Antigen' positive follicles at the end of the culture period was similar between slow-freezing and vitrification.Slow-freezing and vitrification result in similar morphological integrity after cryopreservation, a similar estradiol release in culture, and similar rates of follicular proliferation and apoptosis after culture.