Development of Uric Acid Sensor Using Copper Oxide and Silver Nanoparticles Immobilized SMSMS Fiber Structure-Based Probe
Development of Uric Acid Sensor Using Copper Oxide and Silver Nanoparticles Immobilized SMSMS Fiber Structure-Based Probe
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DOI：
10.1109/tim.2020.2998876
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发表时间：
2020-11-01
影响因子：
5.6
通讯作者：
Kumar, Santosh
中科院分区：
文献类型：
--
作者：
Agrawal, Niteshkumar;Saha, Chinmoy;Kumar, Santosh
利用局域表面等离子体共振（LSPR）技术制备了一种新型的氧化铜纳米颗粒（CuO-NPs）和银纳米颗粒（AgNPs）固定的SM-MM-SM-MM-SM（SMSMS）在线马赫-曾德尔干涉仪（MZI）光纤传感器，并进行了实验验证。AgNPs和CuO-NPs用于改善传感器的生物相容性和灵敏度。通过紫外可见分光光度计、HR-TEM、SEM和EDX对纳米颗粒（NPs）和固定化探针进行了表征，显示出上级现有尿酸（UA）传感器的性能。所提出的优化的基于结构的光纤传感器（OFS）表现出优异的特异性，灵敏度和检测限（LoD）的检测UA。此外，所开发的传感器在非常宽的范围内表现出线性轮廓：1）检测血清中UA的浓度为10 μ M-1 mM，而血清中UA的正常范围为100-400 μ M; 2）检测尿中UA的浓度为0.4-10 mM，而尿中UA的正常范围为1.5- 4.4mM。所开发的传感器具有显著的优点，例如沿着其高选择性、可重复使用性、再现性和最低的LoD，超过了对存在于血清和尿液中的UA的检测的多相容性。所提出的AgNPs和CuO-NPs固定和尿酸酶功能化的SMSMS结构的LSPR传感器具有更好的兼容性，是潜在的候选人，用于医疗应用。
Fabrication and experimental validation of a newly developed copper oxide nanoparticles (CuO-NPs) and silver nanoparticles (AgNPs) immobilized SM-MM-SM-MM-SM (SMSMS) in-line Mach-Zehnder interferometer (MZI) fiber structure-based sensor using localized surface plasmon resonance (LSPR) technique are reported in this article. The AgNPs and CuO-NPs are used to improve the biocompatibility and sensitivity of the sensor. The nanoparticles (NPs) and immobilized probe were characterized by UV-Vis spectrophotometer, HR-TEM, SEM, and EDX, revealing superior performance over the existing uric acid (UA) sensors. The proposed optimized structure-based optical fiber sensors (OFSs) exhibit excellent specificity, sensitivity, and limit of detection (LoD) for the detection of UA. Moreover, the developed sensor exhibits linear profile over a very wide range: 1) 10 mu M-1 mM for detection over UA presents in serum, while the normal range of UA in serum is 100-400 mu M and 2) 0.4-10 mM for detection over UA presents in urine, while the normal range of UA in urine is 1.5-4.4 mM. In addition, the developed sensor has a distinguished advantage, such as multicompatibility over the detection of UA presents in both serum and urine along with its high selectivity, reusability, reproducibility, and lowest LoD. The proposed AgNPs and CuO-NPs immobilized and the uricase functionalized SMSMS structure-based LSPR sensor has better compatibility and are potent candidates for medical applications.