Silicon nitride surface chemistry: A potent regulator of mesenchymal progenitor cell activity in bone formation
Silicon nitride surface chemistry: A potent regulator of mesenchymal progenitor cell activity in bone formation
复制标题
DOI：
10.1016/j.apmt.2017.05.005
复制
发表时间：
2017-12
影响因子：
8.3
通讯作者：
G. Pezzotti;R. Bock;T. Adachi;A. Rondinella;F. Boschetto;Wenliang Zhu;E. Marin;B. McEntire;B. Bal;O. Mazda
中科院分区：
文献类型：
--
作者：
G. Pezzotti;R. Bock;T. Adachi;A. Rondinella;F. Boschetto;Wenliang Zhu;E. Marin;B. McEntire;B. Bal;O. Mazda
多晶氮化硅（Si 3 N4），通过添加少量的钇和铝氧化物（即，Y2 O3和Al 2 O3），具有独特的可调节的表面化学，导致改善细胞代谢和增强骨形成。基于先前使用骨肉瘤细胞进行的体外矿化研究，本研究检测了各种化学调节的Si 3 N4表面与小鼠间充质祖细胞（KUSA-A1）之间的相互作用。研究发现，不同的加压热处理结合绝热和非绝热冷却可使Si_3N_4烧结体表面部分或全部覆盖不同的Si-Y-O-N化合物。通过非绝热冷却获得主要由硅酸钇（β-Y2 Si 2 O 7）的完全覆盖，而在绝热条件下发生由N-磷灰石（Y10（SiO 4）6 N2）的部分覆盖。这些特殊的阶段被发现在刺激KUSA-A1细胞向成骨细胞的体外分化中特别有效，尽管根据不同的微观机制。两个阶段的最终骨形成量几乎相同。通过评估成骨标志物γ-羧基谷氨酸的浓度（即，Gla-骨钙素）。结果发现，在完全相同的条件下，具有N-磷灰石相的Si 3 N4样品比生物医学钛合金对照高出约45%。同时测量骨吸收标志物Glu-骨钙素（即，与对照组相比，γ-羧基谷氨酸的羧基化不足形式）显示出对这些表面处理的Si 3 N4样品上的破骨细胞生成的显著抑制。骨形成采用原位拉曼显微探针光谱和X射线激光显微镜进行评估。这两种独立的分析技术一致发现，与生物医用钛合金相比，表达的羟基磷灰石增加了80%。这项研究表明，表面处理的氮化硅可能有一个强大的合成代谢，分化，和抗凋亡的成骨细胞在体外的影响，并同时抑制破骨细胞的作用。鉴于进一步的研究，Si 3 N4可能代表一种新的治疗方案，用于骨骼疾病和生理调节骨生长过程的工程植入物。
Polycrystalline silicon nitride (Si3N4), sintered with the addition of minor fractions of yttrium and aluminum oxides (i.e., Y2O3and Al2O3), possesses uniquely adjustable surface chemistry that results in improved cell metabolism and enhanced bone formation. Building upon previousin vitromineralization studies using osteosarcoma cells, this study examined interactions between various chemically modulated Si3N4surfaces and murine mesenchymal progenitor cells (KUSA-A1). It was discovered that various pressurized thermal-treatments coupled with adiabatic and non-adiabatic cooling of sintered Si3N4samples resulted in partial or full coverage of their surfaces with different Si–Y–O–N compounds. Full coverage by mostly yttrium silicate (β-Y2Si2O7) was obtained by non-adiabatic cooling, whereas partial coverage with N-apatite (Y10(SiO4)6N2) occurred under adiabatic conditions. These peculiar phases were found to be particularly efficient in stimulating thein vitrodifferentiation of KUSA-A1 cells into osteoblasts, although according to different microscopic mechanisms. The final amount of bone formation was nearly identical for both phases. Cell differentiation was monitored by assessing the concentration of the osteogenic marker γ-carboxyglutamate (i.e., Gla-osteocalcin). It was found to be ∼45% higher for Si3N4samples possessing the N-apatite phase than for biomedical titanium alloy controls tested under exactly the same conditions. Concurrent measurements of the bone resorption marker Glu-osteocalcin (i.e., an undercarboxylated form of γ-carboxyglutamate) showed significant inhibition of osteoclastogenesis on these surface-treated Si3N4samples as compared to the controls. Bone formation was assessed usingin situRaman microprobe spectroscopy andex situlaser microscopy. These two independent analytical techniques consistently found an increase of ∼80% in expressed hydroxyapatite when compared to a biomedical titanium alloy. This study suggests that surface-treated Si3N4may have a powerful anabolic, differentiating, and antiapoptotic effect on osteoblastsin vitro, and a concurrent inhibitive action on osteoclastogenesis. Given additional research, Si3N4may represent a new therapeutic solution for bone disorders and for engineered implants that physiologically regulate bone growth processes.