Imaging the division process in living tissue culture cells
Imaging the division process in living tissue culture cells
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DOI：
10.1016/j.ymeth.2005.07.007
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发表时间：
2006-01-01
期刊：
影响因子：
4.8
通讯作者：
Rieder, CL
中科院分区：
文献类型：
--
作者：
Khodjakov, A;Rieder, CL
我们详细介绍了在有丝分裂过程中通过光学显微镜观察活培养体细胞时遇到的一些陷阱。在这种方法中的原则ditficulties产生的必要性之间的妥协，保持健康的细胞，同时实现适当的时间和空间分辨率所需的研究。虽然从lixed细胞收集的数据的质量只受成像系统的质量和标本的光学性质的限制，观察活细胞时的主要限制因素是照射过程中引起的辐射损伤。我们讨论了减少这种损害的实际考虑。并且用于维持细胞的总体健康，同时随后进行多模式或多维光学显微镜检查。(c)2005年爱思唯尔公司All rights reserved.
We detail some of the pitfalls encountered when following live cultured somatic cells by light microscopy during mitosis. Principle ditficulties in this methodology arise from the necessity to compromise between maintaining the health of the cell while achieving the appropriate temporal and spatial resolutions required for the study. Although the quality of the data collected from lixed cells is restricted only by the quality of the imaging system and the optical properties of the specimen, the major limiting factor when viewing live cells is radiation damage induced during illumination. We discuss practical considerations for minimizing this damage. and for maintaining the general health of the cell, while it is being followed by multi-mode or multi-dimensional light microscopy. (c) 2005 Elsevier Inc. All rights reserved.