Regulation of exoprotein gene expression by the Staphylococcus aureus cvfB gene
Regulation of exoprotein gene expression by the Staphylococcus aureus cvfB gene
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DOI：
10.1128/iai.01552-06
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发表时间：
2007-04-01
影响因子：
3.1
通讯作者：
Sekimizu, Kazuhisa
中科院分区：
文献类型：
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作者：
Matsumoto, Yasuhiko;Kaito, Chikara;Sekimizu, Kazuhisa
我们之前报道了金黄色葡萄球菌的cvfB基因（SA1223）是导致这种致病菌毒力的原因。我们在这里展示了cvfB基因调节外蛋白基因的表达。在cvfB基因缺失突变体中，溶血素、dna酶和蛋白酶的产生减少，而蛋白a的表达增加。在cvfB突变体中，调节各种毒力因子表达的agr位点P3启动子转录本RNAIII的数量也减少了。此外，突变体agr位点的P2和P3启动子活性降低。在agr-null突变的遗传背景下，cvfB基因的破坏降低了DNase和蛋白酶的产生水平。此外，cvfB和agr双突变体对家蚕的毒力低于agr突变体。这些结果表明，cvfB基因产物通过agr依赖性和agr非依赖性途径参与毒力因子的表达和致病性。
We previously reported that the cvfB gene (SA1223) of Staphylococcus aureus is responsible for the virulence of this pathogenic bacterium. We show here that the cvfB gene regulates exoprotein gene expression. In a cvfB gene deletion mutant, hemolysin, DNase, and protease production were decreased, whereas protein A expression was increased. The amount of RNAIII, the transcript from the P3 promoter in the agr locus that regulates the expression of various virulence factors, was also reduced in the cvfB mutant. In addition, P2 and P3 promoter activity in the agr locus was decreased in the mutant. Under the genetic background of the agr-null mutation, cvfB gene disruption decreased the production levels of DNase and protease. Moreover, the cvfB and agr double mutant was less virulent than the agr mutant in silkworms. These results suggest that the cvfB gene product contributes to the expression of virulence factors and to pathogenicity via both agr-dependent and agr-independent pathways.