Optimized agroinfiltration and virus-induced gene silencing to study Ve1-mediated Verticillium resistance in tobacco.
Optimized agroinfiltration and virus-induced gene silencing to study Ve1-mediated Verticillium resistance in tobacco.
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DOI：
10.1094/mpmi-06-12-0161-r
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发表时间：
2013-01
期刊：
影响因子：
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通讯作者：
Zhao Zhang;E. Fradin;R. de Jonge;H. P. van Esse;P. Smit;Chun-Ming Liu;B. Thomma
中科院分区：
文献类型：
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作者：
Zhao Zhang;E. Fradin;R. de Jonge;H. P. van Esse;P. Smit;Chun-Ming Liu;B. Thomma
植物免疫受体对病原体效应物的识别通常导致过敏反应（HR）的激活，这是识别发生的位点周围的植物组织的快速和局部细胞死亡。由于其对功能遗传学的瞬时测定的特别顺从性，烟草是茄科植物家族中免疫信号传导的模型。在这里，我们表明，番茄（番茄）免疫受体Ve1和相应的黄萎病菌效应蛋白Ave1的共表达导致HR仅在特定的烟草物种。而HR在烟草中获得，没有这样的反应，在N。本萨米亚纳此外，我们的分析揭示了烟草中的内源性Ve 1直系同源物，因为在Ve 1不存在的情况下Ave 1的表达诱导了HR，而N.结果表明，该菌株对大丽轮枝菌1号生理小种具有一定的抗性。我们进一步报道了在N. tabacum进行Ve1信号传导的功能分析。总的来说，我们的数据表明，N。烟草可作为研究Ve1介导的免疫信号传导的模式植物。
Recognition of pathogen effectors by plant immune receptors often leads to the activation of a hypersensitive response (HR), which is a rapid and localized cell death of plant tissue surrounding the site at which recognition occurs. Due to its particular amenability to transient assays for functional genetics, tobacco is a model for immune signaling in the Solanaceae plant family. Here, we show that coexpression of the tomato (Solanum lycopersicum) immune receptor Ve1 and the corresponding Verticillium effector protein Ave1 leads to HR only in particular tobacco species. Whereas HR is obtained in Nicotiana tabacum, no such response is obtained in N. benthamiana. Furthermore, our analysis revealed an endogenous Ve1 ortholog in Nicotiana glutinosa, as expression of Ave1 in absence of Ve1 induced a HR, and N. glutinosa was found to be resistant against race 1 Verticillium dahliae. We furthermore report the establishment of virus-induced gene silencing in N. tabacum for functional analysis of Ve1 signaling. Collectively, our data show that N. tabacum can be used as a model plant to study Ve1-mediated immune signaling.