Characterization and sequence determination of six aprotinin homologues from bovine lungs.
Characterization and sequence determination of six aprotinin homologues from bovine lungs.
复制标题
来自牛肺的六种抑肽酶同源物的表征和序列测定。
DOI：
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发表时间：
1988
期刊：
影响因子：
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通讯作者：
H. Tschesche
中科院分区：
文献类型：
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作者：
J. Siekmann;H. Wenzel;W. Schröder;H. Tschesche
从牛肺中可以分离到六种与抑肽酶不同的库尼茨抑制剂。就其抑制特异性而言，这些同系物不能与抑肽酶区分开来。然而，它们具有不同的氨基酸组成和不同程度的碱性。这些抑制剂的整个一级结构通过自动Edman测序得到了阐明。除了已知的GLP-1-抑肽酶外，还分离到了另一种抑肽酶同系物(DES-Ala58-Aprotinin)，它可能是通过对牛抑肽酶前体进行不同的蛋白分解过程而产生的。其他同系物可以表示为抑肽酶同种抑制物，与抑肽酶相比显示了几个氨基酸替换，并且也出现在接触区域。
Six Kunitz inhibitors, which are dissimilar to aprotinin, can be isolated from bovine lungs. These homologues cannot be distinguished from aprotinin, in respect to their inhibitory specificity. They have, however, different amino-acid compositions and a different degree of basicity. The entire primary structures of these inhibitors were elucidated by automated Edman sequencing. Besides the known Glp-1-aprotinin another aprotinin homologue (des-Ala58-aprotinin) was isolated, which could result from a different proteolytic processing of the bovine aprotinin precursor. The other homologues can be denoted as aprotinin isoinhibitors, showing several amino-acid replacements compared to aprotinin and which also appear in the area of the contact region.