Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering.
Preparation of poly(ethylene glycol)/polylactide hybrid fibrous scaffolds for bone tissue engineering.
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DOI：
10.2147/ijn.s25297
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发表时间：
2011
影响因子：
8
通讯作者：
Qian Z
中科院分区：
文献类型：
--
作者：
Ni P;Fu S;Fan M;Guo G;Shi S;Peng J;Luo F;Qian Z
聚乳酸（PLA）电纺丝纤维作为骨组织工程支架材料已被广泛报道，但其良好的疏水性限制了其广泛应用。在本研究中，与相应的空白PLA纤维垫相比，两亲性聚乙二醇(PEG)/疏水性PLA纤维支架具有规则和连续纤维的改善形态。制备的聚乙二醇/聚乳酸纤维支架通过提供相互连接的多孔细胞外环境，有利于间充质干细胞（MSC）的附着和增殖。同时，MSCs可以通过间隙孔渗透到纤维支架中，并与周围纤维很好地结合，这对于在组织工程中的良好应用具有重要意义。更重要的是，通过信使核糖核酸定量和蛋白质分析综合评价成骨过程的代表性标志物，电纺丝PEG/PLA复合纤维支架可以促进MSCs向骨相关细胞分化。PEG/PLA纤维支架上的MSCs表现出更好的分化潜力，早期成骨标志物Cbfa-1和中期成骨标志物Col i的信使核糖核酸表达较高。PEG/PLA纤维支架的碱性磷酸酶活性显著提高，表明这些可以促进MSCs向成骨样细胞的分化。此外，晚期成骨分化标志物OCN（骨钙素）和OPN（骨桥蛋白）的信使核糖核酸水平较高，并伴有茜素红S染色阳性，表明PEG/PLA纤维支架在分化的矿化阶段具有更好的成骨诱导成熟。将PEG/PLA支架移植到大鼠大腿肌袋后，对支架周围的炎症细胞及周围组织的生理特性进行了评价，结果表明PEG/PLA支架具有良好的生物相容性。基于良好的细胞反应和体外成骨潜力，以及与周围组织的生物相容性，电纺丝聚乙二醇/聚乳酸纤维支架有望成为骨组织工程中最有前途的候选材料之一。
Polylactide (PLA) electrospun fibers have been reported as a scaffold for bone tissue engineering application, however, the great hydrophobicity limits its broad application. In this study, the hybrid amphiphilic poly(ethylene glycol) (PEG)/hydrophobic PLA fibrous scaffolds exhibited improved morphology with regular and continuous fibers compared to corresponding blank PLA fiber mats. The prepared PEG/PLA fibrous scaffolds favored mesenchymal stem cell (MSC) attachment and proliferation by providing an interconnected porous extracellular environment. Meanwhile, MSCs can penetrate into the fibrous scaffold through the interstitial pores and integrate well with the surrounding fibers, which is very important for favorable application in tissue engineering. More importantly, the electrospun hybrid PEG/PLA fibrous scaffolds can enhance MSCs to differentiate into bone-associated cells by comprehensively evaluating the representative markers of the osteogenic procedure with messenger ribonucleic acid quantitation and protein analysis. MSCs on the PEG/PLA fibrous scaffolds presented better differentiation potential with higher messenger ribonucleic acid expression of the earliest osteogenic marker Cbfa-1 and mid-stage osteogenic marker Col I. The significantly higher alkaline phosphatase activity of the PEG/PLA fibrous scaffolds indicated that these can enhance the differentiation of MSCs into osteoblast-like cells. Furthermore, the higher messenger ribonucleic acid level of the late osteogenic differentiation markers OCN (osteocalcin) and OPN (osteopontin), accompanied by the positive Alizarin red S staining, showed better maturation of osteogenic induction on the PEG/PLA fibrous scaffolds at the mineralization stage of differentiation. After transplantation into the thigh muscle pouches of rats, and evaluating the inflammatory cells surrounding the scaffolds and the physiological characteristics of the surrounding tissues, the PEG/PLA scaffolds presented good biocompatibility. Based on the good cellular response and excellent osteogenic potential in vitro, as well as the biocompatibility with the surrounding tissues in vivo, the electrospun PEG/PLA fibrous scaffolds could be one of the most promising candidates in bone tissue engineering.