Bacterial Communities Associated with Surfaces of Leafy Greens: Shift in Composition and Decrease in Richness over Time
Bacterial Communities Associated with Surfaces of Leafy Greens: Shift in Composition and Decrease in Richness over Time
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DOI：
10.1128/aem.03470-14
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发表时间：
2015-02-01
影响因子：
4.4
通讯作者：
Brurberg, May Bente
中科院分区：
文献类型：
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作者：
Dees, Merete Wiken;Lysoe, Erik;Brurberg, May Bente
叶际被各种各样的细菌和真菌定殖;它窝藏附生植物，以及植物病原菌，甚至人类病原体。然而，人们对绿叶蔬菜上的细菌群落组成如何随着时间的推移而发展知之甚少。从两个种植火箭沙拉（Diplotaxis tenuifolia）和三个种植生菜（莴苣）在挪威的两个农场获得的绿叶叶际的细菌群落进行了分析的Illumina MiSeq为基础的方法。我们发现，细菌丰富的L。在两个农场，对于种植1和3，种植后不久（3周），sativa样品显著大于收获时（种植后5 - 7周）。对于第二次种植，两个地点的细菌多样性保持一致。这表明，对叶片细菌定殖的影响，至少部分必须是季节性驱动的，而不仅仅是由叶片成熟度驱动的。叶际群落的分布在D. tenuifolia和L.收获时的sativa这些物种在同一地点之间的变异表明，叶栖细菌不仅是被动的居民，但与主机，形状有利于特定类群的增长利基相互作用。这项工作有助于我们了解宿主植物特异性微生物群落结构，并显示这些群落在整个植物发育过程中如何变化。
The phyllosphere is colonized by a wide variety of bacteria and fungi; it harbors epiphytes, as well as plant-pathogenic bacteria and even human pathogens. However, little is known about how the bacterial community composition on leafy greens develops over time. The bacterial community of the leafy-green phyllosphere obtained from two plantings of rocket salad (Diplotaxis tenuifolia) and three plantings of lettuce (Lactuca sativa) at two farms in Norway were profiled by an Illumina MiSeq-based approach. We found that the bacterial richness of the L. sativa samples was significantly greater shortly (3 weeks) after planting than at harvest (5 to 7 weeks after planting) for plantings 1 and 3 at both farms. For the second planting, the bacterial diversity remained consistent at the two sites. This suggests that the effect on bacterial colonization of leaves, at least in part must, be seasonally driven rather than driven solely by leaf maturity. The distribution of phyllosphere communities varied between D. tenuifolia and L. sativa at harvest. The variability between these species at the same location suggests that the leaf-dwelling bacteria are not only passive inhabitants but interact with the host, which shapes niches favoring the growth of particular taxa. This work contributes to our understanding of host plant-specific microbial community structures and shows how these communities change throughout plant development.