Phagocytosis Dynamics Depends on Target Shape
Phagocytosis Dynamics Depends on Target Shape
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DOI：
10.1016/j.bpj.2013.07.036
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发表时间：
2013-09-03
影响因子：
3.4
通讯作者：
Cicuta, Pietro
中科院分区：
文献类型：
--
作者：
Paul, Debjani;Achouri, Sarra;Cicuta, Pietro
对吞噬作用的完整理解需要深入了解其生物化学和物理方面。探索吞噬作用的物理机制的方法之一是探测目标特性（例如，尺寸、形状、表面状态、刚度等）影响其吸收。在这里，我们报告了一种基于成像的方法来探索吞噬动力学，这是兼容的实时成像，并可用于验证现有的报告使用固定和染色的细胞。我们从大量的吞噬事件中测量单次事件吞噬时间，以比较目标的大小和形状如何决定它们的吞噬。数据显示，对于球形颗粒，目标尺寸增加，平均吞没时间增加。非球形颗粒的摄取时间数据证实，目标形状比目标大小对吞噬作用起着更主导的作用：偏心率为0.954且比球形小得多的表面积的椭球体比球形目标慢五倍。
A complete understanding of phagocytosis requires insight into both its biochemical and physical aspects. One of the ways to explore the physical mechanism of phagocytosis is to probe whether and how the target properties (e.g., size, shape, surface states, stiffness, etc.) affect their uptake. Here we report an imaging-based method to explore phagocytosis kinetics, which is compatible with real-time imaging and can be used to validate existing reports using fixed and stained cells. We measure single-event engulfment time from a large number of phagocytosis events to compare how size and shape of targets determine their engulfment. The data shows an increase in the average engulfment time for increased target size, for spherical particles. The uptake time data on nonspherical particles confirms that target shape plays a more dominant role than target size for phagocytosis: Ellipsoids with an eccentricity of 0.954 and much smaller surface areas than spheres were taken up five times more slowly than spherical targets.