Regional, cellular, and ultrastructural distribution of N-methyl-D-aspartate receptor subunit 1 in monkey hippocampus.
Regional, cellular, and ultrastructural distribution of N-methyl-D-aspartate receptor subunit 1 in monkey hippocampus.
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猴海马 N-甲基-D-天冬氨酸受体亚基 1 的区域、细胞和超微结构分布。
DOI：
10.1073/pnas.91.2.564
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发表时间：
1994
影响因子：
11.1
通讯作者：
Morrison,JH
中科院分区：
文献类型：
--
作者：
Siegel,SJ;Brose,N;Janssen,WG;Gasic,GP;Jahn,R;Heinemann,SF;Morrison,JH
用抗N-甲基-D-天冬氨酸(NMDAR-1)660-811融合蛋白的单抗，研究了N-甲基-D-天冬氨酸(NMDAR-1)受体亚单位1在猴海马区的区域、细胞和亚细胞分布。数据表明，海马区每个亚区的许多神经元都含有NMDAR-1蛋白，尽管免疫反应的强度和分布在不同的区域、层和细胞隔间有所不同。在CA3透明层，苔藓纤维轴突表达NMDAR-1，可能与先前假设的突触前受体相对应。NMDAR-1标记的突触后轮廓存在于CA3放射层，但在透明层大部分缺失。这种谷氨酸受体亚单位或类别的神经元内分离可能与呈现层状分离并终止于突触后树突树不同部分的传入系统在空间上相关。例如，在CA3锥体细胞中，NMDA受体在远端顶树突(放射层)中是突触后的，在大鼠中，NMDA依赖的长时程增强是由联合/连合传入介导的，而在近端顶树突(透明层)则不存在，而NMDA非依赖的长时程增强是由苔藓纤维输入介导的。
The regional, cellular, and subcellular distributions of N-methyl-D-aspartate (NMDA) receptor subunit 1, NMDAR-1, were investigated in monkey hippocampus by using a monoclonal antibody directed against a fusion protein corresponding to aa 660-811 of NMDAR-1. The data indicate that many neurons in each subfield of the hippocampus contain NMDAR-1 protein, although the intensity and distribution of immunoreactivity varied across regions, strata, and cellular compartments. In stratum lucidum of CA3, mossy fiber axons were immunoreactive for NMDAR-1, which may correspond to previously hypothesized presynaptic receptors. NMDAR-1-labeled postsynaptic profiles were present in stratum radiatum of CA3 but were largely absent from stratum lucidum. Such intraneuronal segregation of glutamate receptor subunits or classes may be spatially correlated with afferent systems that exhibit laminar segregation and terminate in different portions of the postsynaptic dendritic tree. For example, in CA3 pyramidal cells, NMDA receptors are postsynaptic in distal apical dendrites (stratum radiatum) where NMDA-dependent long-term potentiation in rats is mediated by associational/commissural afferents, and are absent from proximal apical dendrites (stratum lucidum), where NMDA-independent long-term potentiation is mediated by the mossy fiber input.