Escherichia coli K88ac fimbriae expressing heat-labile and heat-stable (STa) toxin epitopes elicit antibodies that neutralize cholera toxin and STa toxin and inhibit adherence of K88ac fimbrial E. coli.
Escherichia coli K88ac fimbriae expressing heat-labile and heat-stable (STa) toxin epitopes elicit antibodies that neutralize cholera toxin and STa toxin and inhibit adherence of K88ac fimbrial E. coli.
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表达热不稳定和热稳定 (STa) 毒素表位的大肠杆菌 K88ac 菌毛会引发中和霍乱毒素和 STa 毒素并抑制 K88ac 菌毛大肠杆菌粘附的抗体。
DOI：
10.1128/cvi.00251-10
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发表时间：
2010
期刊：
影响因子：
--
通讯作者：
Zhang,Weiping
中科院分区：
文献类型：
--
作者：
Zhang,Chengxian;Zhang,Weiping
产肠毒素大肠杆菌（ETEC）菌株是人类和动物腹泻病的主要原因。细菌粘附素和热不稳定（LT）和热稳定（ST）肠毒素是ETEC腹泻的毒力决定因素。据信，诱导抗粘附素免疫以抑制细菌粘附和诱导抗毒素免疫以消除毒素活性的疫苗将提供针对ETEC的广谱保护。在这项研究中，ETEC菌毛粘附素被用作表达粘附素-毒素融合抗原的LT和STa的平台，以诱导抗毒素和抗粘附素免疫。LT毒素B亚基表位（LTP 1，8LCSEYRNTQIYTIN 21）和STa类毒素表位（5CCELCCNPQCAGCY 18）分别嵌入大肠杆菌FaeG主要亚基中。coliK 88 ac菌毛。收获构建的K88 ac-毒素嵌合菌毛并用于兔免疫。免疫兔产生抗K88 ac、抗LT和抗STa抗体。此外，诱导的抗体不仅抑制K88 ac菌毛E的粘附。colito猪小肠上皮细胞也能中和霍乱毒素和STa毒素。本研究的数据表明，表达LT和STa表位抗原的K88 ac菌毛可诱导产生中和性抗毒素抗体和抗粘附素抗体，并提示E.大肠杆菌菌毛可作为开发广谱ETEC疫苗的平台。
EnterotoxigenicEscherichia coli(ETEC) strains are a major cause of diarrheal disease in humans and animals. Bacterial adhesins and heat-labile (LT) and heat-stable (ST) enterotoxins are the virulence determinants in ETEC diarrhea. It is believed that vaccines inducing anti-adhesin immunity to inhibit bacterial adherence and anti-toxin immunity to eliminate toxin activity would provide broad-spectrum protection against ETEC. In this study, an ETEC fimbrial adhesin was used as a platform to express LT and STa for adhesin-toxin fusion antigens to induce anti-toxin and anti-adhesin immunity. An epitope from the B subunit of LT toxin (LTP1,8LCSEYRNTQIYTIN21) and an STa toxoid epitope (5CCELCCNPQCAGCY18) were embedded in the FaeG major subunit ofE. coliK88ac fimbriae. Constructed K88ac-toxin chimeric fimbriae were harvested and used for rabbit immunization. Immunized rabbits developed anti-K88ac, anti-LT, and anti-STa antibodies. Moreover, induced antibodies not only inhibited adherence of K88ac fimbrialE. colito porcine small intestinal enterocytes but also neutralized cholera toxin and STa toxin. Data from this study demonstrated that K88ac fimbriae expressing LT and STa epitope antigens elicited neutralizing anti-toxin antibodies and anti-adhesin antibodies and suggested thatE. colifimbriae could serve as a platform for the development of broad-spectrum vaccines against ETEC.