Repair of bone defects using a new biomimetic construction fabricated by adipose-derived stem cells, collagen I, and porous beta-tricalcium phosphate scaffolds
Repair of bone defects using a new biomimetic construction fabricated by adipose-derived stem cells, collagen I, and porous beta-tricalcium phosphate scaffolds
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DOI：
10.1177/1535370213505827
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发表时间：
2013-10
影响因子：
3.2
通讯作者：
Pei Yang;Xin Huang;Chunsheng Wang;X. Dang;Kunzheng Wang
中科院分区：
文献类型：
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作者：
Pei Yang;Xin Huang;Chunsheng Wang;X. Dang;Kunzheng Wang
具有多谱系分化能力的脂肪干细胞（ASC）已被证明是体外和体内骨再生的替代候选细胞。这表明它们可能是修复骨缺损的潜在候选者。我们试图展示未分化兔脂肪干细胞 (rASC) 的新型仿生结构与 I 型胶原水凝胶封装的完全互连的多孔 β-磷酸三钙 (β-TCP) 支架在兔半径临界尺寸缺损的再生中的用途。制备兔子左侧半径的临界尺寸缺损，并用 rASC/胶原蛋白 I/β-TCP 支架复合材料或胶原蛋白 I/β-TCP 支架复合材料插入。术后 4、8 和 12 周通过组织学、放射线照片、微型 CT、发射计算机断层扫描 (ECT)、荧光染料标记、蛋白质印迹和机械测试来评估结果。植入十二周后，通过放射学、组织学和生物力学检查评估，皮质骨和髓腔的存在证实了缺损几乎完全修复。生物材料的生物降解可能归因于细胞外液体溶解以及细胞介导的吞噬作用。我们的研究表明，I 型胶原蛋白凝胶包裹的多孔 β-TCP 支架中的 rASC 数量较多，可增强临界尺寸缺损的成骨作用。我们希望对基于 rASC 的骨组织工程的临床应用获得新的见解。
Adipose derived stem cells (ASCs) with multilineage differentiation capacities have been demonstrated as an alternative cell candidate for in vitro and in vivo bone regeneration. This suggests that they may be a potential candidate to repair the bone defects. We attempted to demonstrate the use of new biomimetic constructions of undifferentiated rabbit adipose-derived stem cells (rASCs) with fully interconnected porous beta-tricalcium phosphate (β-TCP) scaffolds encapsulated by collagen I hydrogel in the regeneration of a critical-sized defect of rabbit radii. Critical-sized defects in the left radii of rabbits were prepared and inserted with rASCs/collagen I/β-TCP scaffold composites or collagen I/β-TCP scaffold composites. The results were evaluated by histology, radiographs, micro-CT, Emission Computed Tomography (ECT), fluorochrome labeling, western blot, and mechanical testing at 4, 8, and 12 weeks postsurgery. Twelve weeks after implantation, the defects were almost completely repaired as confirmed by the presence of the cortical bone and medullary cavity, which was evaluated through radiologic, histologic, and biomechanical examination. Biodegradation of the biomaterials may be attributed to extracellular liquid dissolution together with cell-mediated phagocytosis. Our study shows that a greater number of rASCs in the porous β-TCP scaffold encapsulated by collagen I gel enhanced osteogenesis in critical-sized defects. We hope to garner new insight into the engineering of rASCs-based bone tissue for clinical application.