The binding, internalization, and release of thymocytes by thymic nurse cells.
The binding, internalization, and release of thymocytes by thymic nurse cells.
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DOI：
10.1006/cimm.1993.1114
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发表时间：
1993-05
影响因子：
4.3
通讯作者：
D. Philp;M. Pezzano;Y. Li;C. Omene;W. Boto;J. Guyden
中科院分区：
文献类型：
--
作者：
D. Philp;M. Pezzano;Y. Li;C. Omene;W. Boto;J. Guyden
我们实验室最近的研究已经描述了SV 40转化的胸腺保育细胞（TNC）系SVT-II 2的发展，该细胞系保持了体外内化胸腺细胞的能力。SVT-II 2细胞显示出仅结合和内化α β TCR+、CD 4 + CD 8+胸腺细胞群的亚群。此外，SVT-II 2细胞表达细胞表面I类和II类MHC抗原。这些数据与表明跨国公司可能在胸腺教育方面发挥作用的报告相一致。在这份报告中，我们使用扫描电子显微镜，透射电子显微镜，和长期视频显微镜研究结合，内化和释放胸腺细胞的跨国公司。这些实验的结果表明，内化事件是选择性的和动态的。这个过程似乎涉及两种细胞类型之间的程序化合作，并以释放选定的胸腺细胞而终止。虽然在胸腺细胞表面表型没有变化，检测到作为其与跨国公司在体外的相互作用的结果，超过90%的仍然是可行的48小时的孵育期后。
Recent studies in our laboratory have described the development of the SV40-transformed thymic nurse cell (TNC) line SVT-II2, that maintains the ability to internalize thymocytes in vitro. SVT-II2 cells were shown to bind and internalize a subset of the alpha beta TCR+, CD4+CD8+ thymocyte population exclusively. Also, SVT-II2 cells express cell surface class I and class II MHC antigens. These data are consistent with reports that suggest that TNCs may have a role in thymic education. In this report, we used scanning electron microscopy, transmission electron microscopy, and long-term video microscopy to study binding, internalization, and release of thymocytes by TNCs. The results of these experiments showed the internalization event to be selective and dynamic. The process appears to involve programmed cooperation between the two cell types that terminates with the release of selected thymocytes. Although no changes in thymocyte cell surface phenotype were detected as a result of their interaction with TNCs in vitro, over 90% remained viable after a 48-hr incubation period.