Identification of rat Tcra-V 4 and 8 gene products by monoclonal antibodies and cDNA sequence.
Identification of rat Tcra-V 4 and 8 gene products by monoclonal antibodies and cDNA sequence.
复制标题
通过单克隆抗体和 cDNA 序列鉴定大鼠 Tcra-V 4 和 8 基因产物。
DOI：
10.1007/bf00189236
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发表时间：
1994
期刊：
影响因子：
3.2
通讯作者：
Hünig,T
中科院分区：
文献类型：
--
作者：
Torres-Nagel,NE;Giegerich,G;Gold,DP;Hünig,T
用Va区和钙区特异的寡核苷酸进行聚合酶链式反应，从2个能检测克隆分布的T细胞表位的单抗G99和G177(分别为N61.5和P2.3)反应的大鼠T细胞系中扩增出c DNA。用小鼠T细胞受体α(TCRA)V4和V8基因片段特异的引物RVa48扩增出片段，但不能与检测到其他Va家族的寡核苷酸结合。来自N61.5(EMBL登录号X75648)的TcrA-V8核苷酸序列与小鼠TcrA-V8.4有88%的相似性，来自P2.3(EMBL登录号X75647)的TcrA-V4与小鼠Tcra-V4片段有90%的相似性。Northern blotting仅在G177阳性T细胞中检测到Va8 mRNA，在G99阳性T细胞中仅检测到Va4 mRNA。
cDNA from 2 rat T-cell lines reactive with monoclonal antibodies G99 and G177 (lines N61.5 and P2.3 respectively), which detect clonally distributed T-cell epitopes, was amplified by the polymerase chain reaction using Va- and Ca-region-specific oligonucleotides. Fragments were obtained with the primer RVa48, which is specific for mouse T-cell receptor a (Tcra)V4andV8gene segments, but not with oligonucleotides detecting other Va families. TheTcra-V8nucleotide sequence from N61.5 (EMBL accession number X75648) expressed a productively arranged element with 88% similarity to mouseTcra-V8.4, and theTcra-V4element derived from P2.3 (EMBL accession number X75647) was 90% similar to a mouseTcra-V4segment. Va8 mRNA was detected by northern blotting only in G177-positive T cells, and Va4 mRNA only in G99-positive T cells.