From Inert Storage to Biological Activity-In Search of Identity for Oxidized Cholesteryl Esters.
From Inert Storage to Biological Activity-In Search of Identity for Oxidized Cholesteryl Esters.
复制标题
DOI：
10.3389/fendo.2020.602252
复制
发表时间：
2020
影响因子：
5.2
通讯作者：
Miller YI
中科院分区：
文献类型：
--
作者：
Gonen A;Miller YI
胆固醇的酯化反应是一种在器官和组织之间储存和运输大量胆固醇并避免过量细胞胆固醇毒性的普遍机制。胆固醇酯（CEs）用于运输和储存，因此是惰性的，它存在于循环脂蛋白和细胞内脂滴的疏水核心中。然而，如果胆固醇被酯化成多不饱和脂肪酸并经过氧化修饰，则ce的惰性特性会发生显著变化。ce的合成后氧化修饰是由专门的酶介导的，其中主要是脂氧合酶和自由基氧化。本文综述了氧化CE （OxCE）产品的复杂曲目，显示出各种环境依赖的生物活性。氧化的脂肪酰基链可以水解和再酯化，从而将氧化的部分转化为磷脂（PLs）， OxPLs与OxCE具有不同的生物活性。质谱技术的进步和新的抗oxce抗体的发展使得验证人类动脉粥样硬化病变和血浆中oxce的存在和量化水平成为可能。本文讨论了测定血浆中氧ce水平作为一种新的生物标志物测定方法来评估心血管疾病的发生风险和治疗效果的前景。
Esterification of cholesterol is a universal mechanism to store and transport large quantities of cholesterol between organs and tissues and to avoid toxicity of the excess of cellular cholesterol. Intended for transport and storage and thus to be inert, cholesteryl esters (CEs) reside in hydrophobic cores of circulating lipoproteins and intracellular lipid droplets. However, the inert identity of CEs is dramatically changed if cholesterol is esterified to a polyunsaturated fatty acid and subjected to oxidative modification. Post-synthetic, or epilipidomic, oxidative modifications of CEs are mediated by specialized enzymes, chief among them are lipoxygenases, and by free radical oxidation. The complex repertoire of oxidized CE (OxCE) products exhibit various, context-dependent biological activities, surveyed in this review. Oxidized fatty acyl chains in OxCE can be hydrolyzed and re-esterified, thus seeding oxidized moieties into phospholipids (PLs), with OxPLs having different from OxCEs biological activities. Technological advances in mass spectrometry and the development of new anti-OxCE antibodies make it possible to validate the presence and quantify the levels of OxCEs in human atherosclerotic lesions and plasma. The article discusses the prospects of measuring OxCE levels in plasma as a novel biomarker assay to evaluate risk of developing cardiovascular disease and efficacy of treatment.
登录
查看更多内容
影响因子：
4
作者：
Gibson MS;Domingues N;Vieira OV
通讯作者：
Vieira OV
影响因子：
3.7
作者：
Choi SH;Wiesner P;Almazan F;Kim J;Miller YI
通讯作者：
Miller YI
DOI：
10.1007/978-3-030-14265-0_1
发表时间：
2019-01-01
期刊：
DIRECT MECHANISMS IN CHOLESTEROL MODULATION OF PROTEIN FUNCTION
影响因子：
--
作者：
Fantini, Jacques;Epand, Richard M.;Barrantes, Francisco J.
通讯作者：
Barrantes, Francisco J.
影响因子：
56.9
作者：
BROWN, MS;GOLDSTEIN, JL
通讯作者：
GOLDSTEIN, JL
影响因子：
4.8
作者：
Fluiter, K;Sattler, W;van Berkel, TJC
通讯作者：
van Berkel, TJC