Induction of activating transcription factor 3 after different sciatic nerve injuries in adult rats
Induction of activating transcription factor 3 after different sciatic nerve injuries in adult rats
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DOI：
10.1080/02844310701318288
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发表时间：
2007-01-01
影响因子：
--
通讯作者：
Dahlin, Lars B.
中科院分区：
文献类型：
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作者：
Kataoka, Kazuya ;;Kanje, Martin;Dahlin, Lars B.
通过激活转录因子 3 (ATF3)（一种神经损伤的神经元标记）进行染色，通过免疫细胞化学对大鼠坐骨神经压碎或横断（再生抑制）后的神经元和雪旺细胞进行检查。 ATF3 免疫反应性在三天后在神经元中达到峰值，然后在挤压后 12 周内逐渐消退至正常。脊髓神经元的反应持续时间较长，并随着时间的推移而下降，但横断后背根神经节（DRG）的反应却没有，这表明感觉和运动神经元的调节存在差异。 ATF3 表达在雪旺细胞中更为明显，并且在横断后保持更长的时间，这意味着再生轴突在某种程度上产生了减少雪旺细胞中 ATF3 表达的信号。然而，即使在没有修复的横断后（不与再生轴突接触），远端雪旺细胞中 ATF3 的表达也会随着时间的推移而下降，这表明再生轴突并不完全是下调的原因。这些发现对于何时值得重建神经损伤具有临床意义。
Staining by activating transcription factor 3 (ATF3), a neuronal marker of nerve injury, was examined by immunocytochemistry in neurons and Schwann cells after crush or transection (regeneration inhibited) of rat sciatic nerve. ATF3 immunoreactivity peaked in neurons after three days and then gradually subsided to normal within 12 weeks after the crush. The response lasted somewhat longer and declined over time in spinal cord neurons but not in those of dorsal root ganglia (DRG) after transection, indicating a differential regulation of sensory and motor neurons. ATF3 expression was more pronounced in Schwann cells, and remained longer after transection, implying that to some extent regenerating axons produce signals that reduce ATF3 expression in Schwann cells. However, even after transection without repair (no contact with regenerating axons), ATF3 expression in Schwann cells in the distal segment decreased over time suggesting that regenerating axons are not entirely responsible for the down-regulation. These findings have clinical implications on when it is worthwhile to reconstruct nerve injuries.