Second-site reversion of a structural defect in bacteriophage T4 lysozyme.
Second-site reversion of a structural defect in bacteriophage T4 lysozyme.
复制标题
噬菌体 T4 溶菌酶结构缺陷的第二位点逆转。
DOI：
10.1096/fasebj.10.1.8566537
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Poteete,AR
中科院分区：
文献类型：
--
作者：
Bouvier,SE;Poteete,AR
使用聚合酶链式反应对编码噬菌体 T4 溶菌酶的质粒携带基因进行诱变，该基因具有结构突变 Tyr161-Ala。将诱变的基因插入专门的噬菌体 λ 克隆载体中，该载体必须获得功能性溶菌酶基因才能形成噬菌斑。选择突变型溶菌酶的功能变体。获得了三个补偿性第二位点回复突变体：Thr152-Met、Lys43-De 和 Thr151-Ala。这些突变的影响是根据之前 T4 溶菌酶的结构和遗传学研究来解释的。-Bouvier, S. E., Poteete, A. R. 噬菌体 T4 溶菌酶结构缺陷的第二位点回复。FASEBJ。 10, 159‐163 (1996)
A plasmid‐borne gene encoding bacteriophage T4 lysozyme with a structural mutation, Tyr161‐Ala, was mutagenized by the use of polymerase chain reaction. The mutagenized gene was inserted into a specialized bacteriophage λ cloning vector that must acquire a functional lysozyme gene in order to form plaques. Functional variants of the mutant lysozyme were selected. Three compensatory second‐site revertants were obtained: Thr152‐Met, Lys43‐De, and Thr151‐Ala. The effects of these mutations are interpreted in light of previous structural and genetic studies of T4 lysozyme.—Bouvier, S. E., Poteete, A. R. Second‐site reversion of a structural defect in bacteriophage T4 lysozyme.FASEBJ. 10, 159‐163 (1996)