Microscopy and supporting data for osteoblast integration within an electrospun fibrous network.
Microscopy and supporting data for osteoblast integration within an electrospun fibrous network.
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DOI：
10.1016/j.dib.2015.10.009
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发表时间：
2015-12
期刊：
影响因子：
1.2
通讯作者：
Barber AH
中科院分区：
文献类型：
--
作者：
Stachewicz U;Qiao T;Rawlinson SC;Veiga Almeida F;Li WQ;Cattell M;Barber AH
该数据文章包含与Stachewicz等人的题为《用于骨再生的细胞与电纺PLGA纳米纤维膜相互作用的3D成像》的研究文章相关的数据。本文利用纤维直径分布直方图分析了聚(d，L-丙交酯-乙交酯酸)电纺纤维在介质和空气中的降解行为。我们还描述了使用聚焦离子束(FIB)显微镜和扫描电子显微镜(SEM)的“切片和查看”断层扫描技术中使用的步骤，并详细介绍了图像分析以获得成骨细胞与电纺纤维网络结合的3D重建。提供了关于电纺纳米纤维膜内细胞生长的量化的进一步支持数据和详细信息。
This data article contains data related to the research article entitled “3D imaging of cell interactions with electrospun PLGA nanofiber membranes for bone regeneration” by Stachewicz et al.. In this paper we include additional data showing degradation analysis of poly(d,l-lactide-co-glycolide acid) (PLGA) electrospun fibers in medium and air using fiber diameter distribution histograms. We also describe the steps used in “slice and view” tomography techniques with focused ion beam (FIB) microscopy and scanning electron microscopy (SEM) and detail the image analysis to obtain 3D reconstruction of osteoblast cell integration with electrospun network of fibers. Further supporting data and detailed information on the quantification of cell growth within the electrospun nanofiber membranes is provided.