Collagen nerve conduits--assessment of biocompatibility and axonal regeneration.
Collagen nerve conduits--assessment of biocompatibility and axonal regeneration.
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DOI：
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发表时间：
2005
影响因子：
1
通讯作者：
F. Stang;H. Fansa;G. Wolf;G. Keilhoff
中科院分区：
文献类型：
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作者：
F. Stang;H. Fansa;G. Wolf;G. Keilhoff
神经缺损的桥接仍是周围神经外科的一个主要问题。作为自体神经移植物的替代，周围神经组织工程的重点是生物相容性导管来重建神经。这种非神经导管由于缺乏通过产生生长因子和细胞引导分子促进再生的活的施万细胞而不能支持在较大间隙上的再生。这个问题可以通过将培养的雪旺细胞植入合适的支架中来克服。在本实验中，我们测试了I/III型胶原蛋白管作为潜在的神经引导基质。通过光镜、荧光显微镜和扫描电镜观察不同植入时间后的血运重建、耐受性和雪旺细胞沉降情况。在术后第5天至第7天，导管完全血运重建，并与宿主组织良好整合。植入的雪旺细胞在导管内表面粘附、存活和增殖。然而，与使用自体移植物的对照相比，使用这些胶原蛋白/雪旺细胞导管桥接成年Wistar大鼠坐骨神经的2 cm间隙导致令人失望的再生。从这些结果中，我们得出结论，生物人工神经导管的足够的生物相容性是一个必要的先决条件，然而，它仍然只是周围神经再生的几个重要参数之一。
Bridging of nerve gaps is still a major problem in peripheral nerve surgery. Alternatively to autologous nerve grafts tissue engineering of peripheral nerves focuses on biocompatible conduits to reconstruct nerves. Such non-neural conduits fail to support regeneration over larger gaps due to lacking viable Schwann cells that promote regeneration by producing growth factors and cell guiding molecules. This problem may be overcome by implantation of cultivated Schwann cells into suitable scaffolds. In the present experiments we tested a collagen type I/III tube as a potential nerve guiding matrix. Revascularization, tolerance and Schwann cell settlement were evaluated by light, fluorescence and scanning electron microscopy after different implantation times. The conduits were completely revascularized between day 5 and 7 post-operatively and well integrated into the host tissue. Implanted Schwann cells adhered, survived and proliferated on the inner surface of the conduits. Nevertheless, bridging a 2 cm gap of the sciatic nerve of adult Wistar rats with these collagen/Schwann cell conduits led to a disappointing regeneration compared to controls with autologous grafts. From these results, we conclude that a sufficient biocompatibility of bioartificial nerve conduits is a necessary prerequisite, however, it remains only one of several parameters important for peripheral nerve regeneration.