Comparison of the proteomes of three yeast wild type strains: CEN.PK2, FY1679 and W303.
Comparison of the proteomes of three yeast wild type strains: CEN.PK2, FY1679 and W303.
复制标题
DOI：
10.1002/cfg.94
复制
发表时间：
2001
影响因子：
--
通讯作者：
Fey, S J
中科院分区：
文献类型：
--
作者：
Rogowska-Wrzesinska, A;Larsen, P M;Blomberg, A;Gorg, A;Roepstorff, P;Norbeck, J;Fey, S J
在基因功能分析项目期间创建的酵母缺失菌株经常显示出取决于所使用的遗传背景的显著表型差异。这些结果表明CEN.PK2、FY1679和W303野生型菌株之间存在重要的分子差异。为了证实这些差异，我们使用二维凝胶电泳比较了CEN.PK2、FY1679和W303菌株之间的蛋白质表达水平，并通过质谱分析鉴定了选定的蛋白质。我们已经发现FY1679和W303菌株彼此之间比CEN.PK2菌株更相似。这项研究确定了62种菌株之间差异表达的蛋白质，并为解释CEN.PK2，FY1679和W303菌株中观察到的酵母突变体表型提供了有价值的数据来源。
Yeast deletion strains created during gene function analysis projects very often show drastic phenotypic differences depending on the genetic background used. These results indicate the existence of important molecular differences between the CEN.PK2, FY1679 and W303 wild type strains. To characterise these differences we have compared the protein expression levels between CEN.PK2, FY1679 and W303 strains using twodimensional gel electrophoresis and identified selected proteins by mass spectrometric analysis. We have found that FY1679 and W303 strains are more similar to each other than to the CEN.PK2 strain. This study identifies 62 proteins that are differentially expressed between the strains and provides a valuable source of data for the interpretation of yeast mutant phenotypes observed in CEN.PK2, FY1679 and W303 strains.