Intracellular protein tyrosine phosphorylation of adherent human macrophages on adsorbed fibronectin.
Intracellular protein tyrosine phosphorylation of adherent human macrophages on adsorbed fibronectin.
复制标题
吸附纤连蛋白上粘附的人巨噬细胞的细胞内蛋白酪氨酸磷酸化。
DOI：
10.1016/s0142-9612(02)00489-1
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发表时间：
2003
期刊：
影响因子：
14
通讯作者：
Liu,Yiping
中科院分区：
文献类型：
--
作者：
Kao,WeiyuanJohn;Liu,Yiping
Fibronectin (FN) was pre-adsorbed onto physicochemically distinct substrates: polyethyleneglycol-based networks or tissue culture polystyrene (TCPS). The role of these substrates in modulating FN-mediated intracellular protein tyrosine phosphorylation and cell adhesion was analyzed with human primary blood derived macrophages. Although macrophage adhesion on both FN-pre-adsorbed TCPS and networks was similarly dependent on protein tyrosine kinase (PTK) and protein serine/threonine kinase (PSK), the compensation between PTK and PSK, and the involvement of signaling molecules (such as protein kinase C (PKC) isoforms) were distinct between the substrates. The pattern and the extent of tyrosine phosphorylation of several proteins (i.e. ∼70, ∼44, ∼30kDa) were differentially regulated by PKCs. FN-derived peptides were employed to probe this material-dependency in macrophage adhesion and tyrosine phosphorylation. The PHSRN domain in the peptide sequence was predominant in mediating this substrate-dependent FN signaling event. We conclude that the tyrosine phosphorylation and the cross talk between PTK and PSK are modulated by FN and the substrate onto which the protein is adsorbed.
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影响因子：
2.9
作者：
N. Ward;C. O'brian
通讯作者：
N. Ward;C. O'brian
由人小梁网细胞粘附到细胞外基质介导的信号转导。
DOI：
10.1006/exer.1999.0806
发表时间：
2000
期刊：
Experimental eye research.
影响因子：
--
作者：
Zhou,L;Cheng,EL;Rege,P;Yue,BY
通讯作者：
Yue,BY
影响因子：
4.4
作者：
Valledor, AF;Xaus, J;Celada, A
通讯作者：
Celada, A
影响因子：
14
作者：
Kao, WYJ;Lee, D
通讯作者：
Lee, D
影响因子：
2.9
作者：
UGAROVA, TP;ZAMARRON, C;PLOW, EF
通讯作者：
PLOW, EF