The transcription factor SlSHINE3 modulates defense responses in tomato plants
The transcription factor SlSHINE3 modulates defense responses in tomato plants
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DOI：
10.1007/s11103-013-0117-1
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发表时间：
2014-01-01
影响因子：
5.1
通讯作者：
Levy, Maggie
中科院分区：
文献类型：
--
作者：
Buxdorf, Kobi;Rubinsky, Gilad;Levy, Maggie
角质层在植物与病原体及其周围环境的相互作用中起着重要的作用。角质层既是抵御物理压力和病原体的物理屏障，也是植物防御反应的化学威慑和激活剂。番茄植株角质层的产生受多种转录因子的调控，其中包括拟南芥WIN/SHN3的同源基因SlSHINE3。在这里，我们利用SLSHINE3过表达(SlSHN3-OE)和沉默(Slshn3-RNAi)品系以及slSHN3的直接靶标SlCyP86A69(Slcyp86A69)中的一个突变体来分析叶片角质层、角质含量和组成在番茄对坏死性叶部病原菌Botrytis cinerea和生物营养性细菌Xanthomonas campestris PV的防御反应中的作用。口水症。我们发现，SLSHN3主要在番茄果实的表皮中表达，它也影响番茄的叶片角质层，因为SlSHN3-OE叶片组织的形态变化导致了有光泽、矮小和透气的叶片。与野生型叶片相比，SLSHN3-OE植株叶片角质单体积累增加38%，而SLshn3-RNAi和Slcyp86A69植株叶片角质单体含量分别减少40%和70%。SLSHN3的过表达导致了对灰霉病和野油菜白粉病的抗性。与角质层通透性和致病相关基因PR1a和AOS表达升高有关。进一步的分析表明，感染B.cinerea的SLshn3-RNAi植株比野生型植株对B.cinerea更敏感，产生更多的过氧化氢。SLSHN3-OE、Slcyp86A69、Slshn3-RNAi和野生型植物的角质单体含量和组成不同，从SLSHN3-OE植物中提取的角质单体改变了野生型植物中致病相关基因的表达。
The cuticle plays an important role in plant interactions with pathogens and with their surroundings. The cuticle acts as both a physical barrier against physical stresses and pathogens and a chemical deterrent and activator of the plant defense response. Cuticle production in tomato plants is regulated by several transcription factors, including SlSHINE3, an ortholog of the Arabidopsis WIN/SHN3. Here we used a SlSHINE3-overexpressing (SlSHN3-OE) and silenced (Slshn3-RNAi) lines and a mutant in SlCYP86A69 (Slcyp86A69)-a direct target of SlSHN3-to analyze the roles of the leaf cuticle and cutin content and composition in the tomato plant's defense response to the necrotrophic foliar pathogen Botrytis cinerea and the biotrophic bacterial pathogen Xanthomonas campestris pv. vesicatoria. We showed that SlSHN3, which is predominantly expressed in tomato fruit epidermis, also affects tomato leaf cuticle, as morphological alterations in the SlSHN3-OE leaf tissue resulted in shiny, stunted and permeable leaves. SlSHN3-OE leaves accumulated 38 % more cutin monomers than wild-type leaves, while Slshn3-RNAi and Slcyp86A69 plants showed a 40 and 70 % decrease in leaf cutin monomers, respectively. Overexpression of SlSHN3 resulted in resistance to B. cinerea infection and to X. campestris pv. vesicatoria, correlated with cuticle permeability and elevated expression of pathogenesis-related genes PR1a and AOS. Further analysis revealed that B. cinerea-infected Slshn3-RNAi plants are more sensitive to B. cinerea and produce more hydrogen peroxide than wild-type plants. Cutin monomer content and composition differed between SlSHN3-OE, Slcyp86A69, Slshn3-RNAi and wild-type plants, and cutin monomer extracted from SlSHN3-OE plants altered the expression of pathogenesis-related genes in wild-type plants.