Eukaryotes in soil aggregates across conservation managements: Major roles of protists, fungi and taxa linkages in soil structuring and C stock
Eukaryotes in soil aggregates across conservation managements: Major roles of protists, fungi and taxa linkages in soil structuring and C stock
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DOI：
10.1016/j.soilbio.2021.108463
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发表时间：
2021-10
影响因子：
9.7
通讯作者：
Elisa Pellegrino;G. Piazza;T. Helgason;L. Ercoli
中科院分区：
文献类型：
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作者：
Elisa Pellegrino;G. Piazza;T. Helgason;L. Ercoli
保护性农业对土壤有机碳稳定的促进作用依赖于土壤团聚体。团聚保护SOC和创造异质微生境托管不同的土壤生物群，反过来又促进团聚。长期试验研究了大豆-小麦轮作中耕作与施氮的相互作用，研究了小团聚体（sM）和封闭微团聚体（mM）内真核生物群落的多样性、组成和结构。使用高通量Illumina测序，我们发现（i）不同的真核生物多样性响应管理强化不同的土壤团聚体和土壤深度;（ii）保守的核心群落组成的真核生物跨CA处理和团聚体在表面和次表层;（iii）不同的影响耕作真核生物群落结构sM和mM沿着土壤剖面根据N的可用性;（iv）原生生物和真菌与sM和mM的量以及它们的SOC含量的正相关;（v）在表面层，mM中的域内和域间网络（真核生物和真核生物-原核生物）的复杂性比sM中的复杂性更强。总的来说，我们的研究结果首次表明，原生生物与真菌一起在土壤结构和碳循环中发挥重要作用，并且Cercozoa代表了土壤生物群聚集网络中的枢纽。
The stabilization of soil organic carbon (SOC) promoted by conservation agriculture (CA) depends on soil aggregation. Aggregation protects SOC and creates heterogeneous microhabitats hosting diverse soil biota which in turn promote aggregation. A long-term experiment, studying the interaction of tillage with nitrogen (N) fertilization on a soybean-wheat rotation, was used to investigate eukaryotic community diversity, composition, and structure within small macroaggregates (sM) and occluded microaggregates (mM). Using high-throughput Illumina sequencing, we found (i) a different eukaryote diversity response to management intensification across soil aggregates and soil depths; (ii) a conserved core community composition of eukaryotes across CA treatments and aggregates at surface and subsurface layers; (iii) a different effect of tillage on eukaryotic community structure in sM and mM along the soil profile according to N availability; (iv) a positive association of protists, and fungi with the amount of sM and mM, and their SOC content; (v) a stronger complexity of within- and cross-domain networks (eukaryotes and eukaryotes-prokaryotes) in mM than in sM at surface layer. Overall, our findings demonstrate for the first time that protists together with fungi play major roles in soil structuring and C cycling, and that Cercozoa represent hubs in soil biota aggregate networks.