Serologic and molecular characterization of a human monoclonal rheumatoid factor derived from rheumatoid synovial cells.
Serologic and molecular characterization of a human monoclonal rheumatoid factor derived from rheumatoid synovial cells.
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源自类风湿滑膜细胞的人单克隆类风湿因子的血清学和分子特征。
DOI：
10.1002/art.1780330820
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发表时间：
1990
影响因子：
--
通讯作者：
Larrick,JW
中科院分区：
文献类型：
--
作者：
Robbins,DL;Kenny,TP;Coloma,MJ;Gavilondo-Cowley,JV;Soto-Gil,RW;Chen,PP;Larrick,JW
类风湿因子（RF）在类风湿关节炎（RA）中的分子表征由于其多克隆性而受到阻碍。为了克服这个问题，我们从类风湿性滑膜细胞中产生了单克隆RF -分泌杂交瘤。在分泌RF的杂交瘤中，HAF10分泌的IgM - RF对人IgG具有单特异性。它能很好地与IgG1和IgG2结合，但不能与IgG3和IgG4结合。对其重链和轻链的序列分析表明，它含有一个VH1重链和一个不属于任何已知λ轻链亚群的Vλ轻链，因此可能代表一个新的λ亚群。这些结果表明，来自类风湿滑膜细胞的单克隆IgM - RF的重链和轻链与报道的主要来自混合冷球蛋白血症患者的几种单克隆RF的可变区序列有很大不同。进一步研究来自RA患者的其他单克隆RF有必要精确定义其遗传基础，并进一步了解RA的免疫病理学。
Molecular characterization of rheumatoid factors (RF) in rheumatoid arthritis (RA) has been hampered because of their polyclonality. To overcome this problem, we generated monoclonal RF‐secreting hybridomas from rheumatoid synovial cells. Among the RF‐secreting hybridomas, HAF10 secreted an IgM‐RF that was monospecific for human IgG. It bound well to IgG1 and IgG2, but not to IgG3 and IgG4. Sequence analysis of its heavy and light chains showed that it contained a VH1 heavy chain and a Vλ light chain that did not belong to any known λ light chain subgroup, and therefore, probably represented a new λ subgroup. These results indicated that both the heavy and light chains of a monoclonal IgM‐RF from rheumatoid synovial cells were quite different from the reported variable region sequences of several monoclonal RF derived mainly from patients with mixed cryoglobulinemia. Further studies of additional monoclonal RF from RA patients are warranted to define precisely their genetic basis and to further our understanding of the immunopathology of RA.