A Hydrogel Microneedle Patch for Point-of-Care Testing Based on Skin Interstitial Fluid
A Hydrogel Microneedle Patch for Point-of-Care Testing Based on Skin Interstitial Fluid
复制标题
一种基于皮肤间质液的水凝胶微针贴片
DOI：
10.1002/adhm.201901201
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发表时间：
2020-01-20
影响因子：
10
通讯作者：
Li, Fei
中科院分区：
文献类型：
--
作者：
He, Rongyan;Niu, Yan;Li, Fei
皮肤间质液（ISF）作为床旁检测（POCT）的非常理想的样本资源具有巨大的潜力，其中关键是有效地从ISF收集目标生物标志物。水凝胶微针贴片在ISF提取方面具有很大的前景。然而，在水凝胶微针贴片中以易于施用、快速和无笨重仪器的方式从提取的皮肤ISF中回收靶生物标志物是具有挑战性的。在此，开发了基于ISF的用于POCT的由聚乙烯醇（PVA）和壳聚糖（CS）制成的水凝胶微针贴片。PVA/CS水凝胶的相变特性使微针在干燥时变硬，有利于更容易穿透皮肤。同时，水凝胶的高度多孔的微观结构为ISF的提取提供了良好的溶胀能力，并且PVA的热降解性质使得可以方便地从微针贴片快速有效地回收靶生物标志物。通过成功检测从模拟皮肤收集的典型生物标志物和监测兔皮肤中的葡萄糖水平，进一步证明了该平台用于POCT应用的能力。所开发的水凝胶微针贴片具有提取皮肤ISF和回收生物标志物的优点，因此为基于皮肤ISF的POCT应用提供了强大的平台。
Skin interstitial fluid (ISF) holds great potential as a highly desirable sample resource for point-of-care testing (POCT), where the key is to effectively collect target biomarkers from ISF. Hydrogel microneedle patch has great promise for ISF extraction. However, it is challenging to recover target biomarkers from the extracted skin ISF in hydrogel microneedle patches in an easy-administrated, fast, and bulky instrument-free manner. Herein, a hydrogel microneedle patch made of polyvinyl alcohol (PVA) and chitosan (CS) for POCT based on the ISF is developed. The phase transition property of PVA/CS hydrogel makes the microneedles stiff when dry, which is beneficial to easily penetrate into skin. Meanwhile, the highly porous microstructure of hydrogel offers good swelling ability for extraction of ISF and the thermal degradation property of PVA makes it convenient to quickly and efficiently recover target biomarkers from microneedle patch. The capability of this platform for POCT applications is further demonstrated by successful detections of typical biomarkers collected from the mimetic skin and monitoring the glucose level in rabbit skins during a day. The developed hydrogel microneedle patch holds the advantages of extraction of skin ISF and recovery of biomarkers, thus presenting as a powerful platform for skin ISF-based POCT applications.