A COMPARATIVE LIGHT AND ELECTRON MICROSCOPICAL STUDY OF COMPATIBLE AND INCOMPATIBLE INTERACTIONS BETWEEN XANTHOMONAS-CAMPESTRIS PV CAMPESTRIS AND CABBAGE (BRASSICA-OLERACEA)
A COMPARATIVE LIGHT AND ELECTRON MICROSCOPICAL STUDY OF COMPATIBLE AND INCOMPATIBLE INTERACTIONS BETWEEN XANTHOMONAS-CAMPESTRIS PV CAMPESTRIS AND CABBAGE (BRASSICA-OLERACEA)
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DOI：
10.1016/0885-5765(89)90026-x
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发表时间：
1989-04-01
影响因子：
2.7
通讯作者：
ROBESON, DJ
中科院分区：
文献类型：
--
作者：
BRETSCHNEIDER, KE;GONELLA, MP;ROBESON, DJ
以甘蓝（Brassica oleracea）的黑腐易感品种（Golden Acre）和抗黑腐品种（Early Fuji）的叶片接种黄单胞菌（Xanthomonas campestris pv）。Campestris （108 cfu ml-1）通过水孔穿孔形成的液滴获得。接种组织5天后固定，光镜和透射电镜检查。细菌是在木质部的传导元素中发现的，伴随着密集的纤维物质，似乎阻塞了细胞的整个腔。在亲和性相互作用中，细菌和纤维物质只填充木质部的传导元件，而周围的叶肉组织基本没有细菌存在，相对没有受到损伤。在不相容的相互作用中，细菌和纤维物质也填充了木质部的传导元件，但除此之外，许多细菌存在于细胞间隙中。这与叶肉细胞壁的严重降解和变形、质解和叶绿体肿胀有关。在相容相互作用中，木质部的传导元件似乎包含了裂解的细菌和染色程度较低的纤维物质的组合，而在不相容相互作用中，没有观察到裂解的细菌细胞，纤维物质似乎也没有被降解。讨论了木质部导电元件中堵塞物质的可能性质和来源。细菌在宿主内的密度和分布，以及组织，细胞和亚细胞破坏的性质描述了与抗性反应或黑腐病发病机制的关系。
Leaves of a black-rot-susceptible cultivar (Golden Acre) and of a black-rot-resistant cultivar (Early Fuji) of cabbage (Brassica oleracea) were inoculated with Xanthomonas campestris pv. campestris (108 cfu ml-1) via droplets formed through guttation at the hydathodes. Inoculated tissue was fixed after five days and examined by light and transmission electron microscopy. The bacteria were found within the xylem conducting elements, accompanied by a dense fibrillar material which appeared to occlude the entire lumen of the cells. In the compatible interaction, bacteria and fibrillar material filled only the xylem conducting elements while the surrounding mesophyll tissue remained essentially free from bacteria and was relatively undamaged. Bacteria and fibrillar material also filled xylem conducting elements in the incompatibile interaction, but, in addition, many bacteria were present in the intercellular spaces. This was associated with severe degradation and deformation of mesophyll cell walls, plasmolysis, and swelling of chloroplasts. In the compatible interaction xylem conducting elements contained what appeared to be a combination of lysed bacteria and less intensely stained fibrillar material, whereas in the incompatible interaction lysed bacterial cells were not observed, and the fibrillar material did not appear to be degraded. The possible nature and origin of the plugging material in the xylem conducting elements is discussed. The density and distribution of bacteria within the host, and the nature of tissue, cellular and sub-cellular disruption are described in relation to the resistance reaction or black rot pathogenesis.