Novel approaches to monitor bacterial gene expression in infected tissue and host.
Novel approaches to monitor bacterial gene expression in infected tissue and host.
复制标题
监测受感染组织和宿主中细菌基因表达的新方法。
DOI：
10.1016/s1369-5274(99)00058-2
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Camilli,A
中科院分区：
文献类型：
--
作者：
Lee,SH;Camilli,A
阐明感染过程中复杂和动态的宿主-微生物相互作用需要，除其他事项外，详细了解微生物体内基因表达。最近，荧光和生物发光检测技术的进步，以及重组酶为基础的体内表达技术，使监测体内毒力基因的表达是一个可行的任务。这些技术已被几个实验室采用，以研究组织培养或动物感染模型感染期间，如鼠伤寒沙门氏菌、单核细胞增生李斯特菌、小肠结肠炎耶尔森氏菌和霍乱弧菌等生物体的毒力基因表达的空间和时间模式。
Elucidating the complex and dynamic host–microbe interactions during infection requires, among other things, detailed knowledge of microbial gene expression in vivo. Recently, advances in fluorescence and bioluminescence detection techniques, as well as recombinase-based in vivo expression technology, have rendered monitoring virulence gene expression in vivo a feasible task. These techniques have been adapted by several laboratories to study the spatial and temporal patterns of virulence gene expression by organisms such as Salmonella typhimurium, Listeria monocytogenes, Yersinia entercolitica and Vibrio cholerae during infection of tissue culture or animal models of infection.