Synthesis and characterization of poly(ethylene oxide)/polylactide/polylysine tri-arm star copolymers for gene delivery
Synthesis and characterization of poly(ethylene oxide)/polylactide/polylysine tri-arm star copolymers for gene delivery
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用于基因传递的聚环氧乙烷/聚丙交酯/聚赖氨酸三臂星形共聚物的合成和表征
DOI：
10.1002/pola.28938
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发表时间：
2018
期刊：
影响因子：
--
通讯作者：
Grubbs, Robert B.
中科院分区：
文献类型：
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作者：
Wu, Tianyuan;Cai, Yu;Zhao, Xia;Ngai, Chai K.;Chu, Benjamin;Hsiao, Benjamin;Hadjiargyrou, Michael;Grubbs, Robert B.
采用开环聚合和巯基-二硫键交换相结合的方法合成了两亲性阳离子聚环氧乙烷-聚赖氨酸-聚乳酸（mPEO-S（CKn）-PLA）三臂星星共聚物。mPEO-S（CKn）-PLA共聚物被发现是无细胞毒性的，并且可以有效地将GFP质粒DNA凝聚成纳米尺寸的复合物，如通过动态光散射（DLS）表征的，适合于内吞细胞摄取。体外DNA转染研究表明，两亲性结构能够进行DNA转染和GFP表达。在培养基中加入氯喹进一步提高了DNA转染效率。© 2017 Wiley Periodicals，Inc. J.波利姆科学，A部分：聚合物化学2018，56，635-644
Amphiphilic cationic poly(ethylene oxide)‐S(polylysine)‐poly(d,l‐lactide) (mPEO‐S(CKn)‐PLA) tri‐arm star copolymers were synthesized by a combination of ring opening polymerization (ROP) and a thiol–disulfide exchange. The mPEO‐S(CKn)‐PLA copolymers were found to be non‐cytotoxic and could effectively condense GFP plasmid DNA into nanometer‐sized complexes, as characterized by dynamic light scattering (DLS), suitable for endocytotic cellular uptake.In vitroDNA transfection studies showed that the amphiphilic structure is capable of DNA transfection and GFP expression. Addition of chloroquine into the medium further enhanced the DNA transfection efficiency. © 2017 Wiley Periodicals, Inc. J. Polym. Sci., Part A: Polym. Chem.2018,56, 635–644