Circular RNA profiling reveals circRNA1656 as a novel biomarker in high grade serous ovarian cancer
Circular RNA profiling reveals circRNA1656 as a novel biomarker in high grade serous ovarian cancer
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DOI：
10.5582/bst.2019.01021
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发表时间：
2019-04-01
影响因子：
5.5
通讯作者：
Wang, Min
中科院分区：
文献类型：
--
作者：
Gao, Yan;Zhang, Chuanqi;Wang, Min
环状RNA（CircRNA）是一类内源性非编码RNA，与人类多种疾病尤其是癌症的发病机制密切相关。然而，circRNA在高级别浆液性卵巢癌（HGSOC）中的特征仍然未知。本研究旨在研究circRNA在HGSOC中的表达谱。基于circRNA的circRNA差异表达的表达谱高通量测序鉴定了3例HGSOC标本和3例正常卵巢组织。共发现710个差异表达的circRNA（354个表达上调，356个表达下调）。在HGSOC组织和良性卵巢病变中通过qRT-PCR验证CircRNA测序数据。通过qRT-PCR证实HGSOC组织中7种circRNA（circRNA 385、circRNA 2058、circRNA 3336、circRNA 2606、circRNA 1656、circRNA 1312和circRNA 7474）的差异表达。其中，circRNA 1656显示出最高的倍数变化。采用qRT-PCR验证卵巢癌细胞系中circRNA 1656的表达。为分析circRNA 1656表达与HGSOC临床病理生物学特征的关系，采用qRT-PCR方法检测60例HGSOC组织中circRNA 1656的表达，并与60例卵巢良性病变进行比较。circRNA 1656在HGSOC组织和卵巢癌细胞系中表达下调，并与HGSOC的FIGO分期相关。circRNA 1656有可能成为HGSOC的一种新的肿瘤标志物。
Circular RNA (circRNA) is a class of endogenous non-coding RNAs that are closely related to the pathogenesis of many human diseases, particularly cancer. However, the characterization of circRNAs in high-grade serous ovarian cancer (HGSOC) remains unknown. This stud) aimed to investigate the expression profile of circRNAs in HGSOC. Expression profiles of circRNAs differential expression based on circRNAs High-throughput sequencing were identified in 3 HGSOC specimens and 3 normal ovarian tissues. A total of 710 differentially expressed circRNAs were found (354 expressions up-regulated and 356 expressions down-regulated). CircRNA sequencing data were verified by qRT-PCR in HGSOC tissue and benign ovarian lesions. Differential expression of 7 circRNAs (circRNA385, circRNA2058, circRNA3336, circRNA2606, circRNA1656, circRNA1312 and circRNA7474) in HGSOC tissue was confirmed by qRT-PCR. Among them, circRNA1656 showed the highest fold change. qRT-PCR was used to verify the expression of circRNA1656 in ovarian cancer cell lines. In order to analyze the relationship between circRNA1656 expression and clinical pathological biological characteristics of HGSOC, qRT-PCR was used to verify the expression of circRNA1656 in 60 HGSOC tissues compared with 60 benign ovarian lesions. The expression of circRNA1656 was down-regulated in HGSOC tissues and ovarian cancer cell lines, and correlated with the FIGO stage of HGSOC. circRNA1656 has the potential to serve as a novel tumor marker for HGSOC.