CHARACTERIZATION OF THE METHANOTROPHIC BACTERIAL COMMUNITY PRESENT IN A TRICHLOROETHYLENE-CONTAMINATED SUBSURFACE GROUNDWATER SITE
CHARACTERIZATION OF THE METHANOTROPHIC BACTERIAL COMMUNITY PRESENT IN A TRICHLOROETHYLENE-CONTAMINATED SUBSURFACE GROUNDWATER SITE
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DOI：
10.1128/aem.59.8.2380-2387.1993
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发表时间：
1993-08-01
影响因子：
4.4
通讯作者：
SAYLER, GS
中科院分区：
文献类型：
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作者：
BOWMAN, JP;JIMENEZ, L;SAYLER, GS
从南卡罗来纳州艾肯附近美国能源部萨凡纳河现场M区的13口监测威尔斯井中采集了被三氯乙烯（TCE）和四氯乙烯（PCE）污染的地下水。过滤后的地下水样品富含甲烷，导致25甲烷氧化分离物的隔离。所有分离株的磷脂脂肪酸谱以18：1 omega 8 c（60 - 80%）为主，这是II组甲烷氧化菌的标志性脂质。随后的表型测试表明，大多数菌株是甲基弯菌属的成员，一个分离株是甲基孢囊菌属的成员。大多数甲烷氧化菌菌株表现出可溶性甲烷单加氧酶（sMMO）活性。这是推测表明萘氧化试验，并证实了杂交与基因探针编码的mmoB基因和细胞提取物测定。TCE降解在不同的速率由大多数的sMMO生产菌株，而PCE没有降解。发现萨凡纳河区域M和其他原始和污染的地下水在补充营养物然后接种发孢甲基弯菌OB 3b时支持sMMO活性。在各种地下水中获得的最大sMMO比活性范围为41至67%，与无铜硝酸盐矿物盐介质中获得的最大速率相比。这项研究部分支持了这一假设，土著甲烷氧化菌群落的刺激可以有效地去除氯化脂肪烃从地下网站，去除可以介导的sMMO。
Groundwater, contaminated with trichloroethylene (TCE) and tetrachloroethylene (PCE), was collected from 13 monitoring wells at Area M on the U.S. Department of Energy Savannah River Site near Aiken, S.C. Filtered groundwater samples were enriched with methane, leading to the isolation of 25 methanotrophic isolates. The phospholipid fatty acid profiles of all the isolates were dominated by 18:1omega8c (60 to 80%), a signature lipid for group II methanotrophs. Subsequent phenotypic testing showed that most of the strains were members of the genus Methylosinus and one isolate was a member of the genus Methylocystis. Most of the methanotroph isolates exhibited soluble methane monooxygenase (sMMO) activity. This was presumptively indicated by the naphthalene oxidation assay and confirmed by hybridization with a gene probe encoding the mmoB gene and by cell extract assays. TCE was degraded at various rates by most of the sMMO-producing isolates, whereas PCE was not degraded. Savannah River Area M and other groundwaters, pristine and polluted, were found to support sMMO activity when supplemented with nutrients and then inoculated with Methylosinus trichosporium OB3b. The maximal sMMO-specific activity obtained in the various groundwaters ranged from 41 to 67% compared with maximal rates obtained in copper-free nitrate mineral salts media. This study partially supports the hypothesis that stimulation of indigenous methanotrophic communities can be efficacious for removal of chlorinated aliphatic hydrocarbons from subsurface sites and that the removal can be mediated by sMMO.