Tissue imaging with a stigmatic mass microscope using laser desorption/ionization
Tissue imaging with a stigmatic mass microscope using laser desorption/ionization
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使用激光解吸/电离的斑块显微镜进行组织成像
DOI：
10.1117/12.909898
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
and Yasuhide Naito
中科院分区：
文献类型：
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作者：
Kunio Awazu;Hisanao Hazama;Tomonori Hamanaka;Jun Aoki;Michisato Toyoda;and Yasuhide Naito
已经开发出一种使用激光解吸/电离和多转飞行时间质谱仪的新型柱头质量显微镜 MULTUM-IMG。可以清晰地观察到被间距为 12.7 μm 的细方网格遮挡的结晶紫的像斑离子图像，并且在 20 倍离子光学放大倍数的线性模式下估计的空间分辨率约为 3 μm。以线性模式观察结晶紫和亚甲蓝染色的小鼠大脑和眼睛的组织切片，结晶紫和亚甲蓝的斑点总离子图像与相同切片的光学显微照片非常吻合。特别是，可以以微米范围的空间分辨率清晰地观察到角膜组织的精细结构。虽然使用重复频率为 10 Hz 的激光对全眼部分的斑象离子图像的总测量时间约为 59 分钟，但使用重复频率为 1 kHz 的激光和测量自动化可将测量时间缩短至约 35 秒。本研究开发的柱头质量显微镜应适用于病理学、药代动力学等领域的高空间分辨率、高通量成像质谱分析。
A novel stigmatic mass microscope using laser desorption/ionization and a multi-turn time-of-flight mass spectrometer, MULTUM-IMG, has been developed. Stigmatic ion images of crystal violet masked by a fine square mesh grid with a 12.7 μm pitch were clearly observed, and the estimated spatial resolution was about 3 μm in the linear mode with a 20-fold ion optical magnification. Tissue sections of a brain and eyes of a mouse stained with crystal violet and methylene blue were observed in the linear mode, and the stigmatic total ion images of crystal violet and methylene blue agreed well with the optical photomicrograph of the same sections. Especially, the fine structure in the cornea tissue was clearly observed with a spatial resolution in the range of micrometers. Although the total measurement time of the stigmatic ion image for the whole-eye section was about 59 minutes using a laser with a 10 Hz repetition rate, the measurement time could be reduced to about 35 s using a laser with a 1 kHz repetition rate and automation of measurements. The stigmatic mass microscope developed in this research should be suitable for high-spatial resolution and high-throughput imaging mass spectrometry for pathology, pharmacokinetics, and so on.