2 GENES INVOLVED IN THE PHASE-VARIABLE PHI-C31 RESISTANCE MECHANISM OF STREPTOMYCES-COELICOLOR A3(2)
2 GENES INVOLVED IN THE PHASE-VARIABLE PHI-C31 RESISTANCE MECHANISM OF STREPTOMYCES-COELICOLOR A3(2)
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DOI：
10.1128/jb.177.16.4681-4689.1995
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发表时间：
1995-08-01
影响因子：
3.2
通讯作者：
BUTTNER, MJ
中科院分区：
文献类型：
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作者：
BEDFORD, DJ;LAITY, C;BUTTNER, MJ
天蓝色链霉菌（Streptomycescoelicolor）的噬菌体生长限制（Pgl）系统使其对phi C31及其同源免疫球蛋白产生抗性。通过亚克隆、插入失活、缺失作图等方法对天蓝色菌DNA进行了序列测定和功能分析，确定了Pgl（+）所需的两个基因pglY和pglZ。（噬菌体抗性）表型，pglY和pglZ，其可以被偶联，预测分别编码M（r）为141，000和101，000的蛋白质，这两种蛋白质与其他已知蛋白质都没有显着的相似性，但PglY具有推定的ATP/GTP结合基序。pglY和pglZ基因从单一启动子共转录，该启动子似乎是组成型的，并且不是由噬菌体感染诱导的。
The phage growth limitation (Pgl) system of Streptomyces coelicolor confers resistance to phi C31 and its homoimmune phages, The positions of the pgl genes within a 16 kb clone of S. coelicolor DNA were defined by subcloning, insertional inactivation, and deletion mapping, Nucleotide sequencing and functional analysis identified two genes, pglY and pglZ, required for the Pgl(+) (phage-resistant) phenotype, pglY and pglZ, which mag. be translationally coupled, are predicted to encode proteins with M(r)s of 141,000 and 101,000, respectively, Neither protein shows significant similarity to other known proteins, but PglY has a putative ATP/GTP binding motif. The pglY and pglZ genes are cotranscribed from a single promoter which appears to be constitutive and is not induced by phage infection.