Genetic diversity in Hypericum and AFLP Markers for species-specific identification of H. perforatum L.
Genetic diversity in Hypericum and AFLP Markers for species-specific identification of H. perforatum L.
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DOI：
10.1055/s-2007-993749
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发表时间：
2007-12
期刊：
影响因子：
2.7
通讯作者：
Ryan J. Percifield;J. Hawkins;J. McCoy;M. Widrlechner;J. Wendel
中科院分区：
文献类型：
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作者：
Ryan J. Percifield;J. Hawkins;J. McCoy;M. Widrlechner;J. Wendel
世界上最畅销的药品之一是贯叶连翘（圣约翰草）。尽管其世界性的分布和利用，很少有人知道的关系，生物活性化合物在H。穿孔的植物，它们据称是来自。为了解金丝桃属植物的多样性，特别是栽培金丝桃属植物的多样性，对11种56份金丝桃属植物进行了AFLP分析。建立一种分子方法学，为育种者和监管者提供一种简单、经济、准确的工具来鉴定所谓的H.穿孔材料。利用4对引物组合共扩增出298个多态性标记，其中17个标记在所有的H. perforatum种质，2份仅为H.穿孔。这项研究表明，AFLP不仅可以用来确定密切相关的金丝桃属种质资源的关系，但作为一种工具，通过使用遗传指纹鉴定材料的草药。
One of the top-selling medicinal products worldwide is Hypericum perforatum (St. John's Wort). Despite its cosmopolitan distribution and utilization, little is known regarding the relationship of the bioactive compounds in H. perforatum to the plants from which they are purportedly derived. In this study, amplified fragment length polymorphism (AFLP) analysis of 56 Hypericum accessions, representing 11 species, was conducted to gain a better understanding of diversity within Hypericum species, especially within cultivated accessions of H. perforatum, and to establish a molecular methodology that will provide breeders and regulators with a simple, affordable, and accurate tool with which to identify purported H. perforatum material. Utilizing four primer combinations, a total of 298 polymorphic markers were generated, of which 17 were present in all H. perforatum accessions and 2 were specific to only H. perforatum. This study demonstrates that AFLP can be utilized not only to determine the relationships of closely related Hypericum accessions, but as a tool to authenticate material in herbal remedies through the use of genetic fingerprinting.