Imbalance of Interleukin-17+ T-cell and Foxp3+ Regulatory T-cell Dynamics in Rat Periapical Lesions
Imbalance of Interleukin-17+ T-cell and Foxp3+ Regulatory T-cell Dynamics in Rat Periapical Lesions
复制标题
大鼠根尖周病变中 IL-17 T 细胞和 Foxp3 调节 T 细胞动力学的不平衡
DOI：
10.1016/j.joen.2013.09.033
复制
发表时间：
2014-01-01
影响因子：
4.2
通讯作者：
Haapasalo, Markus
中科院分区：
文献类型：
--
作者：
Yang, Shasha;Zhu, Lingxin;Haapasalo, Markus
前言：白细胞介素17(IL)-17(+)T辅助细胞(Th17)和Foxp3(+)调节性T(Treg)细胞是具有免疫和骨代谢相互作用的CD4(+)T辅助细胞。本研究旨在探讨Th17和Treg细胞在大鼠根尖周病变中的表达动态及其与骨吸收的关系。方法：对28只Wistar大鼠下颌第一磨牙进行实验性牙髓暴露，造成根尖周损伤。分别于第0、7、21、35天处死大鼠。取下颌骨进行显微CT扫描、组织学观察、免疫组织化学、酶组织化学和双重免疫荧光分析。结果：通过三维和二维测量，从第7天到第21天，根尖周围病变的体积和面积明显增加，然后在第21天到35天缓慢扩大。从第7天到第35天，IL-17阳性细胞显著增加。然而，Foxp3阳性细胞一直保持在低水平，直到第21天，然后在第35天急剧增加。IL-17(+)/Foxp3(+)比值和破骨细胞数从第7天到第21天同时增加，到第35天又下降。最后，在第7天和第35天观察到CD4(+)/IL-17(+)Th17和CD4(+)/Foxp3(+)Treg细胞的明显分布。结论：IL-17(+)T细胞和Foxp3(+)Treg细胞动力学失衡可能在根尖周病变的发生发展中起重要作用。
Introduction: Interleukin (IL)-17(+) T-helper (Th17) cells and Foxp3(+) regulatory T (Treg) cells are CD4(+) T-helper cells with reciprocal functions in immunology and bone metabolism. The present study aimed to investigate the expression dynamics of Th17 and Treg cells in rat periapical lesions as well as their correlation with bone resorption. Methods: Experimental pulp exposures were made in the lower first molars of 28 Wistar rats to induce periapical lesions. Rats were killed on days 0, 7, 21, and 35. Mandibles were prepared for micro computed tomography scanning, histologic observation, immunohistochemistry, enzyme histochemistry, and double immunofluorescence analysis. Results: Through 3-dimensional and 2-dimensional measurements, the volume and area of periapical lesions visibly increased from day 7 to day 21 and then expanded slowly between days 21 and 35. IL-17 positive cells markedly increased from day 7 to day 35. However, Foxp3-positive cells remained at low levels until day 21 and then dramatically increased by day 35. The IL-17(+)/Foxp3(+) ratio and number of osteoclasts simultaneously increased from day 7 to day 21 and then decreased on day 35. Finally, the distinct distribution of CD4(+)/IL-17(+) Th17 and CD4(+)/Foxp3(+) Treg cells was observed on days 7 and 35. Conclusions: Our findings imply the imbalance of IL-17(+) T cell and Foxp3(+) Treg cell dynamics in induced periapical lesions, which may play an important role in periapical lesion progression.