Regeneration of emphysematous lungs using gelatin sheets that release basic fibroblast growth factor
Regeneration of emphysematous lungs using gelatin sheets that release basic fibroblast growth factor
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使用释放碱性成纤维细胞生长因子的明胶片再生肺气肿肺
DOI：
10.1007/s00595-022-02465-z
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发表时间：
2022
期刊：
影响因子：
2.5
通讯作者：
Yokomise Hiroyasu
中科院分区：
文献类型：
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作者：
Otsuki Yasuhiro;Go Tetsuhiko;Kato Ayumu;Yokota Naoya;Fujiwara Atsushi;Matsuura Natsumi;Chang Sung Soo;Misaki Noriyuki;Yokomise Hiroyasu
目的碱性成纤维细胞生长因子（bFGF）诱导肺再生和新血管形成。我们进行了这项研究，以证明再生的肺气肿的肺实现明胶片，缓慢释放碱性成纤维细胞生长因子到内脏胸膜在犬model.MethodsPorcine胰腺弹性蛋白酶被用来诱导双侧下肺叶肺气肿的狗。通过开胸术将缓释bFGF明胶片附着于左下叶的脏层胸膜。受试者被分为两组：一组用含有缓释bFGF的明胶片治疗（bFGF+组，n= 5），另一组仅用明胶片治疗（bFGF−组，n= 5）。28天后对受试者实施安乐死，并进行组织学肺评估。结果评价的平均线性截距（MLI）和微血管计数。结果MLI是在bFGF+组明显短于bFGF−组;（110.0 ± 24.38比208.9 ± 33.08 μm;P= 0.0006）。bFGF+和bFGF−组之间的微血管计数没有显著差异（12.20 ± 3.007 vs. 5.35 ± 2.3425;P= 0.075）;然而，bFGF附着的肺中显著高于肺气肿组（12.20 ± 3.007 vs.4.57 ± 0.8896;P= 0.012）结论在犬肺气肿模型中，将缓释bFGF的明胶片贴附于脏胸膜可诱导肺再生和血管化。
PurposeBasic fibroblast growth factor (bFGF) induces regeneration and neovascularization of the lungs. We conducted this study to demonstrate the regeneration of emphysematous lungs achieved by gelatin sheets that slowly release bFGF into the visceral pleura in a canine model.MethodsPorcine pancreatic elastase was used to induce bilateral lower lobe pulmonary emphysema in dogs. Slow-release bFGF gelatin sheets were attached to the visceral pleura of the left lower lobe via thoracotomy. The subjects were divided into two groups: one treated with gelatin sheets containing slow-release bFGF (bFGF+group,n= 5), and the other, treated with only gelatin sheets (bFGF−group,n= 5). The subjects were euthanized after 28 days and histologic lung assessment was performed. The results were evaluated in terms of the mean linear intercept (MLI) and microvessel count.ResultsThe MLI was significantly shorter in the bFGF+group than in the bFGF−group; (110.0 ± 24.38 vs. 208.9 ± 33.08 μm;P= 0.0006). The microvessel count was not significantly different between the bFGF+and bFGF−groups (12.20 ± 3.007 vs. 5.35 ± 2.3425;P= 0.075); however, it was significantly higher in the bFGF-attached lungs than in the emphysema group (12.20 ± 3.007 vs. 4.57 ± 0.8896;P= 0.012).ConclusionsAttaching gelatin sheets with slow-release bFGF to the visceral pleura induced lung regeneration and vascularization in a canine pulmonary emphysema model.