SNARE zippering and synaptic strength.
SNARE zippering and synaptic strength.
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DOI：
10.1371/journal.pone.0095130
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Charlton MP
中科院分区：
文献类型：
--
作者：
Prashad RC;Charlton MP
突触释放神经递质的概率差异很大。我们检验了反式SNARE（可溶性N-乙基马来酰亚胺敏感因子附着蛋白受体）复合物的拉链状态决定初始释放概率的假设。我们测试了这一假设在相位和紧张性突触不同的100-1000倍的神经递质释放的概率。我们注射，突触前，三个梭菌神经毒素结合和切割在不同的网站上VAMP，以确定这些网站是否被封闭的拉链的圈套复合物或开放的蛋白水解攻击。在低刺激条件下，肉毒杆菌B（BoNT/B-LC）的催化轻链片段抑制诱发的释放在相位和强直性突触和切割VAMP;然而，无论是BoNT/D-LC也破伤风神经毒素（TeNT-LC）在这些条件下是有效的。VAMP对BoNT/B-LC的敏感性表明，在静息状态下，相位性和强直性突触的SNARE复合物仅在N-末端部分拉链到大约零层，C-末端暴露。因此，在相位性和强直性突触处存在相同的trans-SNARE复合物的部分拉链状态表明，释放概率不仅仅由trans-SNARE复合物的拉链状态决定，至少到零层。
Synapses vary widely in the probability of neurotransmitter release. We tested the hypothesis that the zippered state of the trans-SNARE (Soluble N-ethylmaleimide-sensitive factor Attachment protein REceptor) complex determines initial release probability. We tested this hypothesis at phasic and tonic synapses which differ by 100-1000-fold in neurotransmitter release probability. We injected, presynaptically, three Clostridial neurotoxins which bind and cleave at different sites on VAMP to determine whether these sites were occluded by the zippering of the SNARE complex or open to proteolytic attack. Under low stimulation conditions, the catalytic light-chain fragment of botulinum B (BoNT/B-LC) inhibited evoked release at both phasic and tonic synapses and cleaved VAMP; however, neither BoNT/D-LC nor tetanus neurotoxin (TeNT-LC) were effective in these conditions. The susceptibility of VAMP to only BoNT/B-LC indicated that SNARE complexes at both phasic and tonic synapses were partially zippered only at the N-terminal end to approximately the zero-layer with the C-terminal end exposed under resting state. Therefore, the existence of the same partially zippered state of the trans-SNARE complex at both phasic and tonic synapses indicates that release probability is not determined solely by the zippered state of the trans-SNARE complex at least to the zero-layer.
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影响因子：
4.8
作者：
Chen, Sheng;Hall, Cherisse;Barbieri, Joseph T.
通讯作者：
Barbieri, Joseph T.
DOI：
10.1126/science.1224492
发表时间：
2012-09-14
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Gao Y;Zorman S;Gundersen G;Xi Z;Ma L;Sirinakis G;Rothman JE;Zhang Y
通讯作者：
Zhang Y
影响因子：
2.5
作者：
Nguyen, PV;Marin, L;Atwood, HL
通讯作者：
Atwood, HL
影响因子：
3.5
作者：
PELLEGRINI, LL;OCONNOR, V;BETZ, H
通讯作者：
BETZ, H
影响因子：
16.2
作者：
Lin, RC;Scheller, RH
通讯作者：
Scheller, RH