CHANGES IN BIOCHEMICAL COMPOSITION OF CELL-WALL OF COTTON FIBER DURING DEVELOPMENT
CHANGES IN BIOCHEMICAL COMPOSITION OF CELL-WALL OF COTTON FIBER DURING DEVELOPMENT
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DOI：
10.1104/pp.59.6.1088
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发表时间：
1977-01-01
期刊：
影响因子：
7.4
通讯作者：
DELMER, DP
中科院分区：
文献类型：
--
作者：
MEINERT, MC;DELMER, DP
本文对棉花纤维细胞壁的组成进行了研究。阿卡拉SJ-1）从伸长的早期阶段（开花后5天）到次生壁形成时期进行研究，使用来自植物上发育的纤维和来自离体培养的胚珠获得的纤维的细胞壁。拉伸棉纤维的细胞壁是一种动态结构。以总细胞壁的重量百分比表示，纤维素、中性糖（鼠李糖、岩藻糖、阿拉伯糖、甘露糖、半乳糖和非纤维素葡萄糖）、糖醛酸和总蛋白质在伸长期的含量发生显著变化。作为分析壁随时间的绝对变化的一种方式，数据也表示为每mm纤维长度的克组分。以这种方式表示的植物生长的纤维，数据表明，细胞壁的厚度是相对恒定的，直到约12天开花后，在这段时间后，它显着增加，直到次生壁纤维素沉积完成。开花后12和16天之间的所有组件的增加有助于每毫米纤维长度的总壁增加。次生壁纤维素的沉积开始于开花后约16天（至少在伸长停止前5天），并持续至开花后约32天。在次生壁纤维素沉积开始时，蛋白质和糖醛酸含量急剧下降。一些个别中性糖的含量在发展过程中的变化，最突出的变化是一个大的增加，非纤维素葡萄糖发生之前的发病次生壁纤维素沉积。甲基化分析表明，这种葡萄糖，至少部分，是3-连接的。与中性糖相反，在纤维发育过程中观察到细胞壁氨基酸组成没有显著变化。来自培养生长的纤维的细胞壁的成分分析表明，这些壁是非常相似的那些来自生长在植物上的纤维，无论是在组合物和在发展过程中的组合物的相对变化。通过对未分级纤维壁的初步甲基化分析，我们对总细胞壁组成和糖键的结果进行了比较，表明棉纤维的初生细胞壁与文献报道的其他双子叶植物和裸子植物的初生细胞壁相似。
The composition of the cell wall of the cotton fiber (Gossypium hirsutum L. Acala SJ-1) was studied from the early stages of elongation (5 days postanthesis) through the period of secondary wall formation, using cell walls derived from fibers developing on the plant and from fibers obtained from excised, cultured ovules. The cell wall of the elongating cotton fiber was shown to be a dynamic structure. Expressed as a weight percent of the total cell wall, cellulose, neutral sugars (rhamnose, fucose, arabinose, mannose, galactose and noncellulosic glucose), uronic acids and total protein undergo marked changes in content during the elongation period. As a way of analyzing absolute changes in the walls with time, data were also expressed as grams component per mm of fiber length. Expressed in this way for plant-grown fibers, the data show that the thickness of the cell wall is relatively constant until about 12 days postanthesis; after this time it markedly increases until secondary wall cellulose deposition is completed. Between 12 and 16 days postanthesis increases in all components contribute to total wall increase per mm fiber length. The deposition of secondary wall cellulose begins at about 16 days postanthesis (at least 5 days prior to the cessation of elongation) and continues until about 32 days postanthesis. At the time of the onset of secondary wall cellulose deposition, a sharp decline in protein and uronic acid content occurs. The content of some of the individual neutral sugars changes during development, the most prominent change being a large increase in noncellulosic glucose which occurs just prior to the onset of secondary wall cellulose deposition. Methylation analyses indicate that this glucose, at least in part, is 3-linked. In contrast to the neutral sugars, no significant changes in cell wall amino acid composition are observed during fiber development. Compositional analyses of cell walls derived from culture-grown fibers indicate that these walls are remarkably similar to those derived from fibers grown on the plant, both in terms of composition and in terms of relative changes in composition during development. A comparison of our results on total cell wall composition and linkages of sugars as determined by a preliminary methylation analysis of unfractionated fiber walls indicates that the primary cell wall of cotton fibers is similar to that of primary cell walls of other dicotyledons and of gymnosperms as reported in the literature.