Trapping DNA with a high throughput microfluidic device
Trapping DNA with a high throughput microfluidic device
复制标题
DOI：
10.1002/elps.201800287
复制
发表时间：
2019-02-01
期刊：
影响因子：
2.9
通讯作者：
Ladd, Anthony J. C.
中科院分区：
文献类型：
--
作者：
Montes, Ryan J.;Butler, Jason E.;Ladd, Anthony J. C.
通过施加压力梯度和相反的电场，可以在微流体通道的入口附近捕获和浓缩长链DNA。捕获的机制涉及DNA垂直于流体流动和电场的迁移。迁移导致通道横截面内DNA的高度不均匀分布，大部分DNA集中在通道壁附近的薄层（10 μ m）中。该高度浓缩的层产生朝向装置入口的电泳通量，尽管在相反方向上有大得多的流体流动。本文通过荧光测量来表征这种方法可以捕获和浓缩DNA的程度。在短时间内（
Long strands of DNA can be trapped and concentrated near the inlet of a microfluidic channel by applying a pressure gradient and an opposing electric field. The mechanism for trapping involves a migration of DNA perpendicular to both the fluid flow and the electric field. Migration leads to a highly nonuniform distribution of DNA within a cross section of the channel, with the bulk of the DNA concentrated in a thin (10 mu m) layer next to the walls of the channel. This highly concentrated layer generates an electrophoretic flux toward the inlet to the device, despite the much larger fluid flow in the opposite direction. In this paper, the extent to which DNA can be trapped and concentrated by this means has been characterized by fluorescence measurements. At short times (