[Biejiajian Pills inhibits hepatoma carcinoma cell vasculogenic mimicry by suppressing RhoA/ROCK signaling pathway].
[Biejiajian Pills inhibits hepatoma carcinoma cell vasculogenic mimicry by suppressing RhoA/ROCK signaling pathway].
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DOI：
10.3969/j.issn.1673-4254.2018.08.16
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发表时间：
2018-07-30
影响因子：
--
通讯作者：
He, Songqi
中科院分区：
文献类型：
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作者：
An, Haiyan;Lin, Junhao;He, Songqi
目的：为观察鳖甲煎丸对肝癌细胞血管生成拟态（VM）的影响，探讨鳖甲煎丸抑制肝癌转移和侵袭的分子机制，将40只雄性SD大鼠随机分为4组，分别给予生理盐水和鳖甲煎丸高、中、低剂量组（2次/d）灌胃，连续4d。从大鼠收集血清用于处理培养的人HCC HepG 2细胞。在细胞与血清和RhoA/ROCK抑制剂Y-27632（P）孵育后24小时，使用图像采集和分析系统检测细胞中的VM形成。结果：鳖甲煎丸大鼠血清能明显抑制HepG 2细胞VM的形成，VM直径明显大于阳性对照组（P < 0.01）; Y-27632可完全抑制HepG 2细胞VM的形成（P < 0.01）。结论：鳖甲煎丸可能通过抑制RhoA/ROCK信号通路及VE-cadherin和PI 3 K的表达，抑制肝癌细胞VM的形成。
OBJECTIVE: To observe effects of Biejiajian Pills on hepatocarcinoma (HCC) cell vasculogenic mimicry (VM) and explore the molecular mechanism by which Biejiajian Pills inhibits HCC metastasis and invasion.METHODS: Forty male SD rats were randomly divided into 4 groups for gastric lavage of normal saline or high, moderate or low doses of Biejiajian Pills (twice daily) for 4 consecutive days. The sera were collected from the rats for treatment of cultured human HCC HepG2 cells. VM formation in the cells was detected using an image acquisition and analysis system 24 h after incubation of the cells with the sera and with the RhoA/ROCK inhibitor Y-27632(P). The expression levels of RhoA and ROCK1 in the cells were detected using Western blotting, and the contents of VE-cadherin and PI3K in the culture supernatant were determined using ELISA.RESULTS: Treatment with the sera from Biejiajian Pills-treated rats significantly inhibited formation of VM in HepG2 cells, and the diameters of VM formed were significantly greater than those in the positive control group (P < 0.01). Y-27632 completely inhibited the formation of VM in HepG2 cells (P < 0.01). Treatments with Biejiajian Pills and Y-27632 both inhibited the expression of RhoA and ROCK1 (P < 0.05) and significantly lowered the contents of VE-cadherin and PI3K in the culture supernatant (P < 0.05).CONCLUSIONS: Biejiajian Pills can inhibit the formation of VM in HCC cells in vitro possibly by inhibiting the RhoA/ROCK pathways and the expressions of VE-cadherin and PI3K.