Dermal CD271+ Cells are Closely Associated with Regeneration of the Dermis in the Wound Healing Process.
Dermal CD271+ Cells are Closely Associated with Regeneration of the Dermis in the Wound Healing Process.
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DOI：
10.2340/00015555-2624
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发表时间：
2017-05
影响因子：
3.6
通讯作者：
Y. Iwata;Y. Hasebe;Seiji Hasegawa;S. Nakata;A. Yagami;K. Matsunaga;K. Sugiura;H. Akamatsu
中科院分区：
文献类型：
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作者：
Y. Iwata;Y. Hasebe;Seiji Hasegawa;S. Nakata;A. Yagami;K. Matsunaga;K. Sugiura;H. Akamatsu
干细胞在伤口愈合中发挥着重要作用。本研究的目的是研究真皮CD 271+细胞在伤口愈合中的作用。在5岁和24周龄的小鼠背部制作全层伤口，并比较伤口闭合的时间过程、CD 271+细胞计数和基因表达水平。在24周龄的小鼠中观察到延迟的伤口愈合。CD 271+细胞增加的高峰在24周龄的小鼠中延迟，并且在5岁的小鼠中创伤组织中生长因子的基因表达水平显著增加。通过荧光激活细胞分选术（流式细胞仪）纯化的真皮CD 271+细胞比CD 271-细胞表达更高的生长因子，表明CD 271+细胞通过产生生长因子发挥重要作用。本研究还研究了慢性皮肤溃疡患者的真皮CD 271+细胞。与健康对照组相比，患者的真皮CD 271+细胞显著减少。因此，真皮CD 271+细胞与伤口愈合密切相关。
Stem cells have recently been shown to play important roles in wound healing. The aim of this study was to investigate the role of dermal CD271+ cells in wound healing. Full-thickness wounds were produced on the backs of 5-year-old and 24-week-old mice, and time-course of wound closure, CD271+ cell counts, and gene expression levels were compared. Delayed wound healing was observed in 24-week-old mice. The peak of CD271+ cell increase was delayed in 24-week-old mice, and gene expression levels of growth factors in wounded tissue were significantly increased in 5-year-old mice. Dermal CD271+ cells purified by fluorescence-activated cell sorting (FACS) expressed higher growth factors than CD271- cells, suggesting that CD271+ cells play important roles by producing growth factors. This study also investigated dermal CD271+ cells in patients with chronic skin ulcers. Dermal CD271+ cells in patients were significantly reduced compared with in healthy controls. Thus, dermal CD271+ cells are closely associated with wound healing.