First Observation of Experimental Plasmodium vivax Infection of Three Malaria Vectors from the Brazilian Amazon
First Observation of Experimental Plasmodium vivax Infection of Three Malaria Vectors from the Brazilian Amazon
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DOI：
10.1089/vbz.2019.2527
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发表时间：
2020-04-07
影响因子：
2.1
通讯作者：
Pereira-da-Silva, Luiz Hidelbrando
中科院分区：
文献类型：
--
作者：
Araujo, Maisa da Silva;Andrade, Alice Oliveira;Pereira-da-Silva, Luiz Hidelbrando
尽管疟疾是亚马逊地区的特有，但对巴西潜在寄生虫载体的敏感性知之甚少。评估蚊子的媒介敏感性将增加我们对寄生虫 - 向量相互作用的理解，并有助于设计媒介控制策略。这项研究评估了三个肛门物种对中肠感染的敏感性，疟原虫是巴西朗多尼亚州的主要疟疾物种。使用膜喂养测定法（MFA）喂食受疟原虫感染的患者的血液喂食到亚噬菌Anopheles apoheles aquasalis，Anopheles Darlingi和Anopheles Deaneorum蚊子。通过微观检查血液涂片和卵囊患病率来估计配子细胞血症，并评估了感染强度。通过显微镜确定卵囊的存在，并确定所有物种的感染率和感染强度。来自六个MFA的数据表明。达令和。 Deaneorum的感染率最高（分别为97％和90％），并产生了类似的中位数卵卵形卵囊（分别为142和123），而AN。 Aquasalis的感染率最小（77％）和卵囊中位数（88）。既定的实验室殖民地。达令和。 deaneorum和对静脉感染的敏感性将有助于对巴西的Vivax载体 - 寄生虫相互作用进行建模。
Although malaria is endemic to the Amazon region, little is known about the susceptibility of potential parasite vectors in Brazil. Assessing the vector susceptibility of Anopheles mosquitoes will increase our understanding of parasite-vector interactions and aid the design of vector control strategies. This study assessed the susceptibility of three Anopheles species to midgut infection by Plasmodium vivax, the predominant malaria species in Rondonia State, Brazil. Blood from P. vivax infected patients was fed to Anopheles aquasalis, Anopheles darlingi, and Anopheles deaneorum mosquitoes using a membrane feeding assay (MFA). Gametocytemia was estimated by microscopic examination of blood smears and oocyst prevalence, and infection intensity was assessed. The presence of oocysts was determined by microscopy, and the infection rates and infection intensity were determined for all species. Data from six MFAs showed that An. darlingi and An. deaneorum exhibited the highest infection rates (97% and 90%, respectively) and developed a similar median number of P. vivax oocysts (142 and 123, respectively), while An. aquasalis exhibited the smallest infection rates (77%) and the median number of oocysts (88). Established laboratory colonies of An. darlingi and An. deaneorum and susceptibility to plasmodial infection would be beneficial for modeling P. vivax vector-parasite interactions in Brazil.