Tobacco transcription factor WRKY4 is a modulator of leaf development and disease resistance
Tobacco transcription factor WRKY4 is a modulator of leaf development and disease resistance
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DOI：
10.1007/s10535-010-0121-0
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发表时间：
2010-09
影响因子：
1.5
通讯作者：
X. Ren;W. D. Huang;W. Z. Li;D. Yu
中科院分区：
文献类型：
--
作者：
X. Ren;W. D. Huang;W. Z. Li;D. Yu
利用RNAi抑制转基因植株，研究了烟草转录因子WRKY4在叶片发育和生物抗逆性中的作用。与载体对照相比，inNtWRKY4RNAi抑制的转基因品系叶片数量更多、宽度更宽，miRNA166和miRNA396的表达水平降低。ntwrky4在水杨酸（SA）胁迫下表达显著，而非生物胁迫下表达不明显。烟草花叶病毒（TMV）侵染后，转基因植株叶片扭曲程度明显高于载体转基因植株，花叶图案明显高于载体转基因植株。载体转化植株中积累的TMV病毒RNA少于转基因植株。结果表明，NtWRKY4参与了WRKY家族成员中少见的叶片形态发生和抗病毒防御。
The role of tobacco transcription factor WRKY4 in leaf development and biotic stress tolerance was studied using RNAi suppressed transgenic plants. The leaves were more numerous and wider inNtWRKY4RNAi suppressed transgenic lines compared to the vector control, while the levels of miRNA166 and miRNA396 were reduced in suppressed lines.NtWRKY4expression was markedly induced in response to salicylic acid (SA), but not to abiotic stresses. When infected by tobacco mosaic virus (TMV), the leaves of the transgenic plants were more twisted and displayed a more obvious mosaic pattern compared to those of vector-transgenic plants. Less TMV viral RNA accumulated in vector-transformed plants than in transgenic plants. The results indicate that NtWRKY4 is involved in leaf morphogenesis and antiviral defense, which is seldom seen in WRKY family members.