Stromal cell-derived factor 1 (SDF-1) and antenatal human B cell lymphopoiesis:: Expression of SDF-1 by mesothelial cells and biliary ductal plate epithelial cells
Stromal cell-derived factor 1 (SDF-1) and antenatal human B cell lymphopoiesis:: Expression of SDF-1 by mesothelial cells and biliary ductal plate epithelial cells
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DOI：
10.1073/pnas.96.15.8585
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发表时间：
1999-07-20
影响因子：
11.1
通讯作者：
Emilie, D
中科院分区：
文献类型：
--
作者：
Coulomb-L'Herminé, A;Amara, A;Emilie, D
趋化因子基质细胞衍生因子1（SDF-1）在体外刺激前B细胞的生长，并且具有破坏的SDF-1基因的小鼠具有异常的胎肝B细胞淋巴细胞生成。SDF-1的生产来源尚未确定。使用抗SDF-1单克隆抗体，我们进行了免疫组织化学研究，在4个人类胚胎和5个胎儿，以确定哪些细胞表达SDF-1蛋白在产前B细胞淋巴细胞生成的网站。所有间皮细胞在发育的所有阶段都含有SDF-1，包括在妊娠早期的胚内内脏胸膜中胚层中。在胎儿肺和肾中，SDF-1由上皮细胞表达，并且还检测到少量表达V前B链的B淋巴前体。在胎肝中，除间皮细胞外，胆管上皮细胞是唯一含有SDF-1的细胞，表达V链的Pre-B细胞丰富且仅位于汇管区边缘，与胆管板上皮细胞紧密接触。它们不与胆管集合管共定位。胆管板上皮细胞和肝脏B细胞淋巴细胞生成在胎儿期表现出平行的发育和消失。这些结果表明，早期B细胞淋巴细胞在内脏胸膜可能触发间皮细胞产生SDF-1，后来到妊娠，胆管板上皮细胞可能支持B细胞淋巴细胞生成，从而发挥类似的作用，在禽法氏囊上皮细胞和胸腺上皮细胞的T细胞淋巴细胞生成。
The chemokine stromal cell-derived factor 1 (SDF-1) stimulates the growth of pre-B cells in vitro, and mice with a disrupted SDF-1 gene have abnormal fetal liver B cell lymphopoiesis. The origin of SDF-1 production has not been determined yet. Using an anti-SDF-1 mAb, we performed immunohistochemical studies in four human embryos and five fetuses to define which cells express the SDF-1 protein at sites of antenatal B cell lymphopoiesis. All mesothelial cells contained SDF-1 at all stages of development, including in the intraembryonic splanchnopleuric mesoderm early into gestation. In fetal lungs and kidneys, SDF-1 was expressed by epithelial cells, and a few B lymphoid precursor, expressing V pre-B chains, were also detected. In the fetal liver, in addition to mesothelial cells, biliary epithelial cells were the only cells to contain SDF-1, Pre-B cells expressing V chains were abundant and exclusively located around the edge of portal spaces, in close contact with biliary ductal plate epithelial cells. They did not colocalize with biliary collecting ducts. Biliary ductal plate epithelial cells and liver B cell lymphopoiesis display a parallel development and disappearance during fetal life. These results indicate that early B cell lymphopoiesis in the splanchnopleura may be triggered by mesothelial cells producing SDF-1, Later into gestation, biliary ductal plate epithelial cells may support B cell lymphopoiesis, thus playing a role similar to that of epithelial cells in the avian bursa of Fabricius, and of thymic epithelial cells for T cell lymphopoiesis.