Polyamine starvation causes disappearance of actin filaments and microtubules in polyamine-auxotrophic CHO cells
Polyamine starvation causes disappearance of actin filaments and microtubules in polyamine-auxotrophic CHO cells
复制标题
多胺饥饿导致多胺营养缺陷型 CHO 细胞中肌动蛋白丝和微管消失
作者：
P. Pohjanpelto;I. Virtanen;E. Hölttä
多胺腐胺、亚精胺和精胺存在于所有细胞中，它们的合成在细胞增殖过程中显著增加1 - 3。然而，它们在细胞代谢中的确切生理作用尚不清楚。解决这个问题的一个潜在的有效方法是分离和研究不能合成多胺的细胞突变体。这种突变体已经从细菌和酵母中获得，但据我们所知，还没有从高等生物中获得。我们在此报道了从中国仓鼠卵巢细胞中分离出的一种多胺缺乏突变体，与相应的多胺缺失型大肠杆菌突变体4不同，该突变体完全依赖多胺进行持续复制。我们进一步表明，从这些细胞的生长培养基中遗漏多胺会导致肌动蛋白丝束和微管消失，但不会影响中间丝的模式。
The polyamines putrescine, spermidine and spermine are found in all cells and their synthesis increases markedly during cell proliferation1–3. However, their precise physiological role in cellular metabolism is not well understood. A potentially fruitful approach to this problem would be isolation and study of cellular mutants unable to synthesize polyamines. Such mutants have been obtained from bacteria3,4 and yeast5,6 but, to our knowledge, not from higher organisms. We report here the isolation of a polyamine-deficient variant from Chinese hamster ovary cells that, unlike the corresponding polyamine-depleted Escherichia coli mutant4, is absolutely dependent on polyamines for continuous replication. We show further that omission of polyamines from the growth medium of these cells causes bundles of actin filaments and microtubules to disappear but does not affect the pattern of the intermediate filaments.