Preparation of wholemount mouse intestine for high-resolution three-dimensional imaging using two-photon microscopy
Preparation of wholemount mouse intestine for high-resolution three-dimensional imaging using two-photon microscopy
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DOI：
10.1111/j.1365-2818.2009.03163.x
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发表时间：
2009-05-01
影响因子：
2
通讯作者：
Naethke, I.
中科院分区：
文献类型：
--
作者：
Appleton, P. L.;Quyn, A. J.;Naethke, I.
三维可视化整体组织结构是验证和整合来自较不复杂系统（如培养细胞）的生物化学、细胞生物学和视觉数据的基础。在这里，我们描述了一种方法来生成完整的小鼠肠道组织的高分辨率三维图像数据。最感兴趣的区域位于该组织内的50至200 μ m之间。由于与散射光、光漂白和球面像差相关的问题，具有这种深度的组织的三维图像数据的质量和有用性受到限制。此外，最高质量的油浸镜头设计用于在最大距离
Visualizing overall tissue architecture in three dimensions is fundamental for validating and integrating biochemical, cell biological and visual data from less complex systems such as cultured cells. Here, we describe a method to generate high-resolution three-dimensional image data of intact mouse gut tissue. Regions of highest interest lie between 50 and 200 mu m within this tissue. The quality and usefulness of three-dimensional image data of tissue with such depth is limited owing to problems associated with scattered light, photobleaching and spherical aberration. Furthermore, the highest-quality oil-immersion lenses are designed to work at a maximum distance of